|
|||||
|
|
Molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis by plasmid analysis and pulsed-field gel electrophoresis |
|
| Authors: | Aktas Zerrin Day Martin Kayacan Cigdem Bal Diren Sukufe Threlfall E John |
| Affiliation: | aIstanbul University, Istanbul Faculty of Medicine, Department Microbiology and Clinical Microbiology, Istanbul, Turkey bLaboratory of Enteric Pathogens, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK cIstanbul University, Cerrahpasa Faculty of Medicine, Department of Pediatrics, Microbiology Lab, Istanbul, Turkey |
| Abstract: | Forty-one paediatric isolates of Salmonella spp. from Cerrahpasa Faculty of Medicine, Istanbul, Turkey, between 2001 and 2004 were examined for susceptibility to various antibiotics and presence of antibiotic resistance genes. Pulsed-field gel electrophoresis and plasmid profiling were used to determine possible genetic relationships among Salmonella enterica subsp. enterica clinical isolates. Plasmids from resistant strains were not transferred by conjugation to recipient Escherichia coli cells. Pulsed-field gel electrophoresis and restriction enzyme digestion analysis of DNA revealed that multidrug-resistant isolates belonged to the same clonal group, characterized by ACSSuT resistotype. Isolates of R-type ACSSuT were positive for the intI gene and possessed a single plasmid of 60 MDa. |
| Keywords: | Salmonella Typhimurium DT 104 ACSSuT Salmonella Enteritidis Plasmid profile analysis |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |