Correlation of hippocampal glucose oxidation capacity and interictal FDG-PET in temporal lobe epilepsy |
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Authors: | Vielhaber Stefan Von Oertzen Joachim H Kudin Alexei F Schoenfeld Ariel Menzel Christian Biersack Hans-Juergen Kral Thomas Elger Christian E Kunz Wolfram S |
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Institution: | Department of Neurology II, University of Magdeburg Medical Center, Magdeburg, Germany. vielhaber@medizin.uni-magdeburg.de |
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Abstract: | PURPOSE: Interictal 18F]fluorodeoxyglucose (FDG) positron emission tomography (PET) demonstrates temporal hypometabolism in the epileptogenic zone of 60-90% of patients with temporal lobe epilepsy. The pathophysiology of this finding is still unknown. Several studies failed to show a correlation between hippocampal FDG-PET hypometabolism and neuronal cell loss. Because FDG is metabolized by hexokinase bound to the outer mitochondrial membrane, we correlated the glucose-oxidation capacity of hippocampal subfields obtained after surgical resection with the corresponding hippocampal presurgical FDG-PET activity. METHODS: In 16 patients with electrophysiologically confirmed temporal lobe epilepsy, we used high-resolution respirometry to determine the basal and maximal glucose-oxidation rates in 400-microm-thick hippocampal subfields obtained after dissection of human hippocampal slices into the CA1 and CA3 pyramidal subfields and the dentate gyrus. RESULTS: We observed a correlation of the FDG-PET activity with the maximal glucose-oxidation rate of the CA3 pyramidal subfields (rp = 0.7, p = 0.003) but not for the regions CA1 and dentate gyrus. In accordance with previous studies, no correlation of the FDG-PET to the neuronal cell density of CA1, CA3, and dentate gyrus was found. CONCLUSIONS: The interictal hippocampal FDG-PET hypometabolism in patients with temporal lobe epilepsy is correlated to the glucose-oxidation capacity of the CA3 hippocampal subfield as result of impaired oxidative metabolism. |
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Keywords: | Temporal lobe epilepsy FDG-PET Mitochondrial oxidative phosphorylation |
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