五子衍宗丸对小鼠记忆力、免疫功能、耐缺氧及耐疲劳的影响

目的:观察五子衍宗丸对小鼠记忆力、免疫功能、耐缺氧及耐疲劳的影响。方法:选用ICR雄性小鼠,五子衍宗丸高、中、低剂量分别按生药计为2.4,1.2,0.6 g·kg-1,ig给药。以Morris水迷宫测定小鼠记忆力和认知能力;碳粒廓清率、迟发型变态反应测定免疫功能;常压缺氧和常温游泳法测定耐缺氧和耐疲劳能力。结果:与对照组比较,五子衍宗丸各剂量组小鼠寻找隐藏平台的逃避潜伏期及总路程明显缩短(P0.05或P0.01);高剂量组廓清指数K值明显增加(P0.01);各剂量组耳肿胀度无明显差异;高、中剂量组耐缺氧时间明显延长(P0.01);高剂量组常温游泳时间有所延长(P0.05);结论:五子衍宗丸具有明显增强小鼠记忆力和空间认知力,提高小鼠耐缺氧、抗疲劳能力的作用;同时可提高非特异性免疫功能,但对细胞免疫功能无明显作用。

Effects of Wuzi Yanzong Pill on Memory,Immune Function, Anti-hypoxia and Anti-fatigue in Mice

Objective: To observe the effects of Wuzi Yanzong Pill on the memory,immune function,anti-hypoxia and anti-fatigue in mice. Method: Wuzi Yanzong Pill was administered to ICR mice at oral doses of crude drug 0.6,1.2,2.4 g ·kg^-1.The learning and memory ability in mice were tested by Morris water maze experiment.Carbon expurgation method and delayed allergy were used to determine the immune function.Fatigue and acute anoxia in mice were produced by swimming and hypoxia under normal pressure respectively and survival time was determined. Result: Compared with control group,the escape latency and swimming distance of all dose groups were shorter (P<0.05 or P<0.01).High dosage increased the expurgatory index(P<0.01)but all dose groups had no effect on swelling of ear.High and middle dosage significantly prolonged the survival time under hypoxia(P<0.01).High dosage prolonged the swimming time of mice(P<0.05). Conclusion: Wuzi Yanzong Pill has remarkable effect on enhancing the ability of learning and memory in ICR mice,improving the tolerance of hypoxia and fatigue,promoting non-specific immune function but has no effect on cellular immune function.