Measurement of the alpha-mannosidase activities in human plasma by a differential assay. |
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| Authors: | S Hirani B G Winchester A D Patrick |
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| Affiliation: | 1. Department of Biochemistry, Queen Elizabeth College (University of London), Campden Hill Road, London W8 7AH U.K.;2. Institute of Child Health (University of London), Guilford Street, London WC1N 1EH U.K. |
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| Abstract: | A differential assay based on their difference in thermal stability has been used to measure the acidic and true intermediate alpha-mannosidases in the plasma of cntrols and individuals homozygous or heterozygous for mannosidosis. The intermediate activity was found to be independent of age, sex or mannosidosis genotype. The acidic alpha-mannosidase did not vary significantly with age or between sexes for groups of the same age. The concentrations of acidic and intermediate alpha-mannosidase showed a positive correlation for adults but not for children. The ratio of acidic to true intermediate alpha-mannosidase might therefore be a useful secondary test for the detection of adult heterozygotes for mannosidosis. |
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| Keywords: | To whom correspondence should be addressed. |
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