安体舒通对自发性高血压大鼠肾小球纤维化的影响

目的观察安体舒通对自发性高血压大鼠（SHR）肾小球纤维化的影响及可能机制。方法16只14周龄雄性SHR随机等分为安体舒通组和对照组，分别以安体舒通30mg／（kg·d）及等量生理盐水灌胃12周，8只同龄雄性SD大鼠作为正常对照组，免疫组化和RT-PCR检测结缔组织生长因子（CTGF）、转化生长因子β1（TGF—β1）的表达；MASSON染色分析测量胶原容积分数（CVF）；碱水解法测定羟脯氨酸（Hypro）含量。结果SHR对照组CVF（7．78±0．28）％]、Hypro（491±35．0）μg／g]，CTGF（14．87±0．73）、TGF—β1（18．31±0．86）表达明显高于正常对照组分别为（4．36±0．41）％，（321±53．8）μg／g，8．40±0．67，12．04±0．71，P〈0．01]；相对于SHR对照组，安体舒通组则显著降低分别为（6．35±1．12）％，（429±53．7）μg／g，13．02±0．92，16．37±1．24，P〈0．05]。结论安体舒通能明显改善SHR肾小球纤维化，其作用可能是通过阻断盐皮质激素受体和抑制CT—GF及TGF-β1的表达而实现的。

Effect of spironolactone on glomerulus fibrosis of spontaneously hypertensive rats

Objective To observe the effect of spironolactone on glomerulus fibrosis of spontane-ously hypertensive rats.Methods 16 fourteen-week-old male SHRs were equivalently random assigned to dium chloride for 12 weeks by gavage.8 same age male SD rats were selected as control group.CTGF and TGF-β1 were determined by immunohistochemistry and RT-PCR.CVF was measured by MASSON staining.The concentration of Hypro was detected by alkaline hydrolysis method.Results The CVF(7.78±0.28)%],Hypro(491±35.0)μg/g],and the expression of TGF-β1(18.31±0.86),CTGF(14.87±0.73)in SHR group were significantly higher than those in SD group(4.36±0.41)%,(321±53.8)μg/g,8.40±0.67,12.04±0.71,P<0.01].Compared with the SHR group,these indices in spironolactone treatment group decreased obviously(6.35±1.12)%,(429±53.7)μg/g,13.02±0.92,16.37±1.24,P<0.05].Conclusions Spironolactone significantly improved glomerulus fdarosis of SHR by blocking mineralocorticoid receptor and suppressing the expression of CTGF and TGF-βl.