缓激肽对C6胶质瘤细胞分泌白细胞介素-6的影响

目的:白细胞介素-6(IL-6)与脑胶质瘤的恶性增殖密切相关,本文探讨缓激肽作用大鼠C6胶质瘤细胞后对IL-6表达的影响。方法:应用逆转录聚合酶链反应(RT-PCR)和放射免疫法分别检测1μmol·L^-1 缓激肽作用下,C6胶质瘤细胞内及细胞培养液中IL-6的表达水平。结果:RT-PCR结果显示缓激肽作用于C6胶质瘤细胞,在不同时相点(0,5,10,15,30,60min)均有IL-6mRNA的表达,各组间比较无显著性差异(P>0.05);放射免疫法结果显示,细胞培养液中未检测到IL-6的表达。结论:缓激肽作用C6胶质瘤细胞60min以内不引起IL-6的表达增强,这为缓激肽临床应用的安全性提供了有利的实验依据。

The Effect of Bradykinin on Secretion of Interleukin-6 in C6 Glioma Cells

Objective: To investigate the effect of bradykinin on the expression of interleukin-6(IL-6) in C6 glioma cells. Methods: Semi-quantitative RT-PCR and radioimmunoassay were used to detect the expression of IL-6 mRNA in C6 glioma cells and IL-6 protein in culture medium from cells treated with 1μ mol/L bradykinin, respectively. Results: Semi-quantitative RT-PCR revealed that IL-6mRNA was detected at different time points (0, 5, 10, 15, 30 and 60 min) in C6 glioma cells after treatment with bradykinin at 1μ mol/L, but there was no significant difference between the treatment group and the control group (P>0.05). Radioimmunoassay revealed that expression of IL- 6 protein wasnot detected in the cell culture medium. Conclusion: There is a close correlation between interleukin-6 (IL-6) expression and malignant proliferation in glioma. Bradykinin fails to evoke an enhancement inthe expression of IL-6 in C6 glioma cells within an hour, which may provide a favorable experimental basis for the clinical application of bradykinin.