罗伯逊易位型21三体永生淋巴细胞株的建立 |
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引用本文: | 杨小康,;徐丽娟,;王飞,;徐忠乐,;杜久伟,;奚娜,;朋云峰. 罗伯逊易位型21三体永生淋巴细胞株的建立[J]. 中国优生与遗传杂志, 2014, 0(12): 31-33 |
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作者姓名: | 杨小康, 徐丽娟, 王飞, 徐忠乐, 杜久伟, 奚娜, 朋云峰 |
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作者单位: | [1]安徽理工大学医学院检验教研室,安徽淮南232000; [2]安徽理工大学生殖医学研究所,安徽淮南232000; [3]中国科学技术大学生命科学院,安徽合肥230000 |
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基金项目: | 安徽理工大学2014年大学生科研项目(ZZl40);国家级大学生创新创业训练计划项目(NO.201310361095);安徽省教育厅基金(K22012A080). |
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摘 要: | 目的建立并鉴定13/21易位与21/21罗伯逊易位21三体永生淋巴细胞株,为21三体罗伯逊易位的遗传学研究提供实验材料。方法筛查获得易位型21三体患者并收集外周血,培养B95-8细胞制备EB病毒感染液,采用EB病毒转化法获得永生淋巴细胞株,对第10、15、20代细胞进行G显带染色体分析。结果筛检出1例罕见的13/21易位与4例121/21罗伯逊易位型21三体患儿并成功建立永生淋巴细胞株,传代至第10、15、20代细胞的核型无显著差异。结论明病毒转化法可用于易位型21三体永生淋巴细胞株的建立,早期转化的细胞可为该病的研究提供实验基础。
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关 键 词: | 21-三体 罗伯逊易位 EB病毒 永生淋巴细胞株 |
Establishment of immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21. |
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Affiliation: | YANG Xiao-kang, XU Li-juan, WANG Fei, XU Zhong-le, DU Jiu-wei, XI Na, PENG Yun-feng. ( 1.Department of Laboratory Medicine, Medical School, Anhui University of Science and Technology; 2.1nstitute of reproductive Medicine, Anhui University of Science and Technology; 3.School of Life Sciences, University of Science and Technology of China, Hefei City 230000, China) |
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Abstract: | Objective. To establish immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21 by Epstein-Barr (EB) virus and conduct karyotype analysis of the cells. Methods: Genetic screening for the Robertsonian translocation type trisomy 21 children; then collection patient' s peripheral blood. Cultivation of B95-8 cells was prepared for EB virus transformation of peripheral blood B lymphocytes. Karyotype analysis was performed for the cultured cells of passages 10, 15, and 20 to evaluate their genetic stability. Results: Screening out a rare case of rob ( 13; 21 ) and 3 cases of rob (21; 21 ) . Altogether 5 immortalized lymphoblastoid cell lines with stable chromosome were obtained successfully from the patients. No chromosomal abnormalities were found in the cultured cells of passages 10, 15, and 20. Conclusion. EB virus can be used in the establishment of immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21, this chromosomal disease is studied preferably with. cells of early passages. |
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Keywords: | Robertsonian translocations Trisomy21 Epstein-Barrvirus B-lymphoblastoid cell lines |
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