CYPl7、CYPlA2酶基因多态性与子宫内膜异位症的关系

目的探讨CYPl7 T-34C、CYPIA2 G-2964A基因多态性与子宫内膜异位症遗传易感性的关系。方法抽取108例确诊为子宫内膜异位症患者及84例对照组女性的静脉血，提取全基因组DNA，分别用聚合酶链式反应（PCR）技术扩增CYPl7 T-34C、CYPIA2 G-2964A多态位点的基因片段，并对其进行酶切，根据酶切结果计算出各等位基因的表型频率，用X2检验及OR值分析其多态性与子宫内膜异位症的关系。结果病例组和对照组CYPl7基因的基因型及等位基因的频率分布比较差异有统计学意义（P〈O.05）。病例组和对照组CYPIA2基因型、等位基因频率的比较差异无统计学意义（P〉0.05）。结论CYPl7 T-34C可能与III、IV期子宫内膜异位症发病风险相关。CYPlA2 G-2964A多态性可能不是子宫内膜异位症独立的危险因素。

Relationship between CYP17, CYP1A2 enzyme gene polymorphism and Endometriosis

Objective： To investigate the relationship between CYP17 T-34C, CYP1A2 G-2964A gene polymorphism and genetic susceptibility to endometriosis. Methods： Take venous blood of 108 cases who have been diagnosed with endometriosis and 84 cases of the control group of women, Extracted total genomic DNA, CYP17 and CYP1A2 fragment were amplified by polymerase chain reaction （PCR） , After PCR amplification, digested the products with different restriction enzyme. Based on the results, calculated for each allele phenotype frequencies, using X2test and OR values to analyze the relationship between CYP17 T-34C gene. CYP1A2 G-2964A Gene polymorphism and genetic susceptibility to endometriosis. Results： CYP17 genotype and allele frequencies between case group and control group, The difference was statistically significant （P〈0.05） , CYP1A2 genotype and allele frequencies between case group and control group. The difference no statistically significant （P〉0.05） . Conclusion： CYP17 T-34C may contribute to the genetic influence on stage III, IV endometriosis development, CYP1A2 G-2964A polymorphism may not be an independent risk factor of endometriosis.