醒脑静合生脉注射液下调大鼠脑出血后脑组织PAR1和AQP4表达

目的研究醒脑静合生脉注射液对大鼠脑出血后脑组织内凝血酶受体-1(PAR1)和水通道蛋白-4(AQP4)表达的影响。方法将40只雄性SD大鼠随机分为脑出血组(ICH)、生理盐水组(NS)、醒脑静合生脉注射液治疗组(XNJSM)、水蛭素治疗组(HIR),每组10只。采用自体不凝血注入法制备脑出血模型。术后72 h取脑组织,HE染色观察各组血肿周围神经细胞形态学改变,免疫组化方法及Western Blotting法检测各组血肿周围脑组织PAR1和AQP4表达。结果脑出血后血肿周围脑组织PAR1、AQP4表达均明显增强,XNJSM组、HIR组脑组织病理形态明显改善,脑组织内PAR1、AQP4的表达减少,与ICH组相比较差异均具有统计学意义(P<0.05)。结论脑出血后PAR1和AQP4两种蛋白的表达增加可能共同参与了脑出血后继发性脑损伤的过程,醒脑静合生脉注射液能够有效抑制大鼠脑出血后PAR1和AQP4蛋白的表达,对脑出血后神经损伤发挥保护作用。

Xingnaojing and Shengmai Injection Down-Regulating the Expressions of PAR1 and AQP4 in Rats After Intracerebral Hemorrhage

Objective To study the effect of Xingnaojing and Shengmai injection （XNJSM） on protease-activated receptors-1 （PAR1） and aquaporin-4 （AQP4） expressions in rat brain tissue after intracerebral hemorrhage （ICH）. Methods The ICH model was induced by injecting blood of itself. Forty male SD rats were randomly divided into ICH group, saline group （NS）, XNJSM group, hirudin group （HIR）, with 10 rats in each group. Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes and the expressions of PAR1 and AQP4 in perihematomal tissue by immunohistochemistry and Western Blotting respectively after 72 hours. Results The expressions of PAR1 and AQP4 inperihematomal tissue increased after ICH,however,compared with ICH group,perihematomal tissue morphological changes improved obviously, and the expressions of PAR1 and AQP4 also decreased significantly between XNJSM group and HIR group （P〈0.05）. Conclusions The increased expressions of PAR1 and AQP4 may induce some brain injury following ICH together, and Xingnaojing and Shengmai injection can alleviate neural injuries after ICH by inhibiting the expressions of PAR1 and AQP4.