Molecular characterization of a clinical Haemophilus parainfluenzae isolate with cefotaxime resistance and decreased susceptibility to fluoroquinolones |
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| Affiliation: | 1. Servicio Antimicrobianos, National Reference Laboratory, Instituto Nacional de Enfermedades Infecciosas-ANLIS “Dr. Carlos G. Malbrán”, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;3. Hospital General de Niños “Dr. Ricardo Gutierrez”, Buenos Aires, Argentina;1. Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina;2. Red Latinoamericana de Vigilancia de la Resistencia a los Antimicrobianos (ReLAVRA);3. Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Buenos Aires, Argentina |
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| Abstract: | We report an H. parainfluenzae clinical isolate resistant to cefotaxime and with decreased susceptibility to ciprofloxacin recovered from a patient with cystic fibrosis. The isolate had elevated MICs of ampicillin (256 mg/L), amoxicillin-clavulanate (8 mg/L), cefuroxime (8 mg/L) and cefotaxime (4 mg/L), and showed a β-lactamase-producing amoxicillin-clavulanic acid-resistant (BLPACR) phenotype. A blaTEM-1 plus five amino acid substitutions in the PBP3 were found: Ser385Thr, Val511Ala, Ile519Val, Asn526Lys and Asp551Leu. MIC of ciprofloxacin was 0.5 mg/L, and substitutions in gyrA (Ser84Tyr) and parC (Ser84Phe) genes were detected. |
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