三氧化二砷对膀胱癌T24细胞增殖及APC基因表达的影响

目的:探讨三氧化二砷（arsenic trioxide，As₂O₃）对人膀胱癌T24细胞增殖及腺瘤性结肠息肉病相关基因（adenomatous polyposis coli，APC）表达的影响。方法:采用2、4、8 μmol/L的As₂O₃处理T24细胞，MTT法检测T24细胞增殖抑制率；脱氧核糖核酸原位末端转移酶标记技术检测细胞凋亡；用RT-PCR及Western blot法分别检测As₂O₃对T24细胞中APC mRNA和蛋白表达的影响。结果:As₂O₃在2~8 μmol/L浓度范围内处理T24细胞72 h可有效抑制细胞增殖（P<0.01）；促进细胞凋亡（P<0.01）；增加APC mRNA和蛋白表达（P<0.01）。结论:在一定浓度范围内（2~8 μmol/L）As₂O₃可以有效抑制T24细胞增殖和诱导其凋亡，其机制可能与上调APC基因的表达有关。

Effect of arsenic trioxide on the proliferation and gene expression of APC in bladder cancer T24 cells

Objective:To observe the effects of arsenic trioxide(As₂O₃) on the proliferation and gene expression of adenomatous polyposis coli(APC) in human bladder cancer T24 cells.Methods:The T24 cells were processed with 2,4 and 8μmol/L of As₂O₃,the proliferation inhibition rate was detected using MTT assay,the apoptosis was detected using the deoxyribose situ terminal transferase labeling,and the expression levels of APC mRNA and protein were detected using RT-PCR and Western blot,respectively.Results:The 2 to 8 μmol/L of As₂O₃ treated T24 cells for 72 h could effectively inhibit the cell proliferation(P<0.01),promote the cell apoptosis(P<0.01),and increase the expression of APC mRNA and protein(P<0.01).Conclusions:As₂O₃ (2~8μmol/L) can effectively inhibit the proliferation,and induce the apoptosis of T24 cells,the mechanism of which may be related to the upregulation of APC gene expression.