Molecular characterization of two rare human G8P[14] rotavirus strains,detected in Italy in 2012 |
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| Institution: | 1. National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy;2. Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy;3. Department of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, Bari, Italy;1. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Virology Department, Pasteur Institute of Iran, Tehran, Iran;1. Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa;2. Centro de Investigação em Saúde de Manhiça (CISM), Manhiça, Mozambique;3. Institute of Hygiene and Tropical Medicine, Lisbon, Portugal;4. Next Generation Sequencing Unit, Department of Medical Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa;5. Biochemistry, Focus Area Human Metabolomics, North-West University, Potchefstroom, South Africa;6. Deltamune (Pty.) Ltd., Lyttelton, Centurion, South Africa;7. Faculdade de Medicina, Universidade Eduardo Mondlane, Maputo, Mozambique;8. Instituto Nacional de Saúde, Maputo, Mozambique |
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| Abstract: | Since 2007, the Italian Rotavirus Surveillance Program (RotaNet-Italy) has monitored the diversity and distribution of genotypes identified in children hospitalized with rotavirus acute gastroenteritis.We report the genomic characterization of two rare human G8P14] rotavirus strains, identified in two children hospitalized with acute gastroenteritis in the southern Italian region of Apulia during rotavirus strain surveillance in 2012.Both strains showed a G8-P14]-I2-R2-C2-M2-A11-N2-T6-E2-H3 genomic constellation (DS-1-like genomic background). Phylogenetic analysis of each genome segment revealed a mixed configuration of genes of animal and zoonotic human origin, indicating that genetic reassortment events generated these unusual human strains. Eight out of 11 genes (VP1, VP2, VP3, VP6, VP7, NSP3, NSP4 and NSP5) of the Italian G8P14] strains exhibited close identity with a Spanish sheep strain, whereas the remaining genes (VP4, NSP1 and NSP2) were more closely related to human strains. The amino acid sequences of the antigenic regions of outer capsid proteins VP4 and VP7 were compared with vaccine and field strains, showing high conservation between the amino acid sequences of Apulia G8P14] strains and human and animal strains bearing G8 and/or P14] proteins, and revealing many substitutions with respect to the RotaTeq? and Rotarix? vaccine strains. Conversely, the amino acid analysis of the four antigenic sites of VP6 revealed a high degree of conservation between the two Apulia strains and the human and animal strains analyzed.These results reinforce the potential role of interspecies transmission and reassortment in generating novel rotavirus strains that might not be fully contrasted by current vaccines. |
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