Analysis of nosocomial Staphylococcus haemolyticus by MLST and MALDI-TOF mass spectrometry |
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| Institution: | 1. Federal Research and Clinical Centre of Physical–Chemical Medicine, Moscow, Russia;2. Federal State Budget Institution Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia;3. Ulyanovsk State Technical University, Ulyanovsk, Russia;1. Lomonosov Moscow State University, Leninskie Gory, 1, Moscow 119991, Russian Federation;2. Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, Moscow 119435, Russian Federation;1. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation;2. S. N. Winogradsky Institute of Microbiology, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russian Federation;3. Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation;4. Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russian Federation;5. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation;6. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation |
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| Abstract: | Coagulase-negative staphylococci (CoNS) are a major component of normal human skin and mucosae flora. However, some species of CoNS can lead to infections in immunocompromised patients and premature newborns. The choice of a rapid and reliable typing method is one of the major problems in the epidemiological monitoring of CoNS, especially Staphylococcushaemolyticus isolates. In this study, we have tested 71 isolates of S. haemolyticus obtained from newborns using the multilocus sequence typing (MLST) and the direct bacterial MALDI-TOF mass spectrometry profiling approaches. To date, there is no standard MLST scheme for investigating the diversity of S. haemolyticus strains. The novel variant of MLST scheme including the tpiA, pta, sh1200, rphE, tphK, mvaK1, and arc loki was tested. The discriminatory power was estimated by the Hunter–Gaston discriminatory index (D) as 0.95. The Composition Correlation Index Matrix (CCI matrix) was calculated to typing the isolates through the analysis of mass spectra; also, the values of the correlation index for different groups of isolates were evaluated. Closely related isolates obtained from the same hospital are characterized by increased values of correlation indices in comparison with these values of isolates collected from various hospitals. The data obtained by both methods allow to describe a clonal structure of S. haemolyticus population and to designate the presence of endemic clones of S. haemolyticus. |
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