Cytolethal distending toxin producing Escherichia coli O157:H43 strain T22 represents a novel evolutionary lineage within the O157 serogroup |
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| Institution: | 1. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary;2. Seqomics Biotechnology Ltd., Mórahalom, Hungary;3. Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary;1. ANSES, Ploufragan-Plouzané Laboratory, Hygiene and Quality of Poultry and Pig Products Unit, Bretagne-Loire University, Ploufragan, France;2. Rennes 1University, Rennes, France;1. APERAM, Research Center, F-62330 Isbergues, France;2. Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France;3. INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France;4. CNRS, UMR5504, F-31400 Toulouse, France;5. Université de Toulouse; UPS, INPT; LAPLACE, 118 Route de Narbonne, F-31062 Toulouse cedex 9, France;6. CNRS, LAPLACE, F-31062 Toulouse, France;7. INRA, UMR1319 Micalis, F-78350 Jouy-en-Josas, France;8. AgroParisTech, UMR Micalis, F-78350 Jouy-en-Josas, France;9. PSL Research University, Chimie ParisTech-CNRS, Institut de Recherche de Chimie Paris, Research Group Physical Chemistry of Surfaces, 75005 Paris, France |
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| Abstract: | Enterohemorrhagic Escherichia coli (EHEC) O157:H7/NM strains are significant foodborne pathogens intensively studied, while other sero- and pathotypes of the O157 serogroup only began to receive more attention. Here we report the first genome sequence of a cytolethal distending toxin (CDT-V) producing E. coli O157:H43 strain (T22) isolated from cattle. The genome consists of a 4.9 Mb chromosome assembled into three contigs and one plasmid of 82.4 kb. Comparative genomic investigations conducted with the core genomes of representative E. coli strains in GenBank (n = 62) confirmed the separation of T22 from the EHEC and enteropathogenic (EPEC) O157 lineages. Gene content based pangenome analysis revealed as many as 261 T22-specific coding sequences without orthologs in EDL933 EHEC O157 prototypic and two phylogenetically related commensal E. coli strains. The genome sequence revealed 10 prophage-like regions which harbor several virulence-associated genes including cdt and heat-labile enterotoxin (LT-II) encoding operons. Our results indicate that the evolutionary path of T22 is largely independent from that of EHEC and EPEC O157:H7/NM strains. Thus, the CDT-producing T22 E. coli O157:H43 strain represents a unique lineage of E. coli O157. |
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