A novel avian-like hepatitis E virus in wild aquatic bird,little egret (Egretta garzetta), in Hungary |
| |
| Institution: | 1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary;2. Department of Medical Microbiology and Immunology, Medical Center, University of Pécs, Pécs, Hungary;3. Department of Pathophysiology, Medical Center, University of Pécs, Pécs, Hungary;4. Hungarian Nature Research Society (HuNaReS), Ajka, Hungary;5. Blood Systems Research Institute, San Francisco, CA, USA;6. University of California, San Francisco, CA, USA;1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary;2. University of Pécs, Pécs, Hungary;3. Blood Systems Research Institute, San Francisco, CA, USA;4. University of California, San Francisco, CA, USA;1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary;2. SHP Ltd., Kaposvár, Hungary;3. Blood Systems Research Institute, San Francisco, CA, USA;4. University of California, San Francisco, CA, USA;1. National Institute for Research and Development in Forestry “Marin Drăcea”, Cluj-Napoca Research Station, 400275 Cluj-Napoca, Romania;2. National Institute for Research and Development in Forestry “Marin Drăcea”, Simeria Research Station, 335900 Simeria, Romania;3. Faculty of Forestry, Technical University in Zvolen, 96053 Zvolen, Slovakia;4. Faculty of Forestry, University of Sarajevo, 71000 Sarajevo, Bosnia and Herzegovina;5. Slovenian Forest Institute, 1000 Ljubljana, Slovenia;6. University of Forestry, 1797 Sofia, Bulgaria;7. National Institute of Research and Development for Biological Sciences – Institute of Biological Research, 400015 Cluj-Napoca, Romania;8. Department Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum, 60325 Frankfurt/Main, Germany;9. National Institute for Research and Development in Forestry “Marin Drăcea”, 077190 Voluntari, Ilfov, Romania;10. Transilvania University of Braşov, Faculty of Silviculture and Forest Engineering, 500123 Braşov, Romania;1. Vrije Universiteit Brussel (VUB), Ecology and Biodiversity, Marine Biology, Pleinlaan 2, 1050 Brussels, Belgium;2. Sokoine University of Agriculture, Department of Biological Sciences, 3038 Morogoro, Tanzania;3. Vrije Universiteit Brussel (VUB), Analytical, Environmental and Geo-Chemistry, Pleinlaan 2, 1050 Brussels, Belgium;4. Sokoine University of Agriculture, Department of Medicine & Public Health, 3021 Morogoro, Tanzania;5. Sokoine University of Agriculture, Department of Microbiology, Parasitology and Immunology, Genome Science Center, 3019 Morogoro, Tanzania |
| |
| Abstract: | Hepatitis E virus (HEV), family Hepeviridae, has public health concerns because of its zoonotic potential; however, the host species spectrum, animal to animal transmissions, the natural chain of hepevirus infections and the genetic diversity of HEV in wildlife especially in birds are less known. Using random amplification and next generation sequencing technology a genetically divergent avian HEV was serendipitously identified in wild bird in Hungary. HEV RNA was detected with high faecal viral load (1.33 × 108 genomic copies/ml) measured by real-time PCR in faecal sample from a little egret (Egretta garzetta). The complete genome of HEV strain little egret/kocsag02/2014/HUN (KX589065) is 6660-nt long including a 18-nt 5′ end and a 103-nt 3′ end (excluding the poly(A)-tail). Sequence analyses indicated that the ORF1 (4554nt/1517aa), ORF2 (1728nt/593aa) and ORF3 (339nt/112aa) encoded proteins of little egret/kocsag02/2014/HUN shared the highest identity (62.8%, 71% and 61.5%) to the corresponding proteins of genotype 1 avian (chicken) HEV in species Orthohepevirus B, respectively. This study reports the identification and complete genome characterization of a novel orthohepevirus distantly related to avian (chicken) HEVs at the first time in wild bird. It is important to recognize all potential hosts, reservoirs and spreaders in nature and to reconstruct the phylogenetic history of hepeviruses. Birds could be an important reservoir of HEV generally and could be infected with genetically highly divergent strains of HEV. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
