|
|||||
|
|
Antigenicity and immunogenicity of hepatitis B virus particles produced by mouse cells transfected with cloned viral DNA |
|
| Authors: | C Pourcel E Sobzack M F Dubois M Gervais J Drouet P Tiollais |
| Institution: | 1. Unité de Recombinaison et Expression Génétique (Inserm U163, CNRS LA 271), France;2. Groupement de Génie Génétique, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France;3. Unité de Recherche sur les Infections Virales, Inserm U.43, Hôpital St Vincent de Paul, 74 Avenue Denfert Rochereau, 75014 Paris, France;4. Centre National de Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France |
| Abstract: | Antigenicity and immunogenicity of the hepatitis B surface antigen (HBsAg) 22-nm particles produced by mouse cells transfected with HBV DNA were studied. Both the a group and the y subtype determinants were present on the particles. Injected into mice the particles induced formation of anti-HBs. An antibody titer of 400 IU/mP, 7 weeks after the primary injection was obtained in one experiment. The affinity constant of these antibodies to human HBsAg particles was about 1 × 108? mol/liter. The anti-HBs antibodies react specifically with both the a group and the y subtype HBV determinants. These results show that these particles have antigenic and immunogenic properties similar to those of the 22-nm particles present in human serum. |
| Keywords: | TTo whom reprint requests should be addressed |
| 本文献已被 ScienceDirect 等数据库收录！ | |