白藜芦醇改善高脂饮食小鼠的糖代谢并棕化腹股沟白色脂肪

目的：观察白藜芦醇治疗对高脂饮食诱导肥胖小鼠的体重，体脂比及血糖代谢的作用，初步探索白藜芦醇对白色脂肪棕化的作用。方法：小鼠高脂饮食（45％热量来自脂肪）8周后随机分为对照组（HFD—Con）和白藜芦醇治疗组（HFD—Res，白藜芦醇400mg／kg），治疗2周后称重、测定糖耐量和计算体脂比例，实时PCR测定腹股沟皮下白色脂肪组织解耦联蛋白-1（UCP-1）和cIDEA基因表达。结果：与HFD．Con组相比，HFD—Res组体重下降明显，糖耐量改善，脂肪组织／体重比无改变；而腹股沟皮下白色脂肪组织解耦联蛋白-1（UCP-1）和CIDEA基因表达升高。结论：白藜芦醇治疗可降低高脂饮食诱导肥胖小鼠体重和改善糖代谢，同时可能促进腹股沟皮下白色脂肪组织棕化。

Resveratrol exerts anti-obesity effects while browning the inguinal white adipose tissue

Objective： To investigate the effects ofresveratrol on the body weight, ratio of white adipose tissue, glucose level and UCP-1, CIDEA mRNA expression in high fat diet-induced obese mice. Methods： Obese mice induced by 8 weeks＇ high fat diet （HFD） were treated with resveratrol at a dose of 400 mg/kg （HFD-Res） or placebo （HFD-Con） for 2 weeks and were weighted. The ratio of white adipose tissue was calculated and glucose metabolism was evaluated by intraperitoneal glucose tolerance test （ipGTT）. The gene expression of UCP-1 and CIDEA was determined by real-time PCR. Results： Compared with HFD-Con, HFD-Res had more weight loss （P〈0.05） while ipGTT was improved after 2 weeks＇ therapy. RT-PCR results showed that the expression of UCP-1 and CIDEA mRNA in the inguinal white adipose tissue was higher in HFD-Res than in HFD-Con （P〈0.05）. Conclusion： Resveratrol has anti-obesity effect and can improve glucose metabolism in high fat diet-induced obese mice. Resveratrol can increase the gene expression of UCP-1 and CIDEA in inguinal white adipose tissue, indicating its potential effect in browning of white adipose tissue.