FISH Preimplantation Diagnosis of Chromosome Aneuploidy in Recurrent Pregnancy Wastage |
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Authors: | F Vidal C Giménez C Rubio C Simón A Pellicer J Santaló J Egozcue |
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Institution: | (1) Unitat de Biologia Cel.lular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain;(2) Unitat de Biologia Cel.lular, Facultat de Ciències, Universitat Autònoma Barcelona, Barcelona, Spain;(3) Instituto Valenciano de Infertilidad (IVI), Valencia, Spain;(4) Departamento de Pediatría, Obstetricia y Ginecologia, Universidad de Valencia, Spain |
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Abstract: | Purpose:
Our purpose was to detect aneuploidy for chromosomes 13, 16,18, 21, 22, X, and Y in preimplantation embryos from patients with a history of unexplained recurrent miscarriage.
Methods:
Three patients with a history of unexplained recurrent spontaneous abortion were included in this study. Embryos were biopsied at the eight-cell stage, individually fixed on slides, and processed for fluorescent in situ hybridization (FISH). A multiple FISH protocol for seven chromosomes pairs (13, 16, 18, 21, 22, X, and Y) has been developed.
Results:
A total of 39 embryos was studied with the multiple FISH protocol developed. Successful analysis of the biopsied embryos was achieved within the time limits usually allowed in a preimplantation diagnosis program. Analysis of the blastomeres showed that 17 embryos were chromosomally normal for the probes used, 16 embryos were aneuploid, and in 6 embryos no informative results were obtained.
Conclusions:
In the patients studied, a large proportion of embryos (41%) exhibited chromosomal abnormalities for the probes used. Preimplantation diagnosis to screen for chromosome abnormalities could be a feasible approach to improve the possibility of successful pregnancy in these couples. |
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Keywords: | aneuploidy fluorescent in situ hybridization miscarriage preimplantation genetic diagnosis |
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