黄芩苷-小檗碱复合物纳米晶的制备和在体肠吸收评价

[目的]研究纳米晶对黄芩苷-小檗碱复合物的肠吸收促进作用.[方法]使用抗溶剂法结合高压均质法制备黄芩苷-小檗碱复合物纳米晶,进行初步体外评价.建立在体单向肠灌流模型,评价纳米晶对复合物吸收的促进效果.[结果]制备了黄芩苷-小檗碱复合物纳米晶.体外评价结果表明,黄芩苷-小檗碱复合物纳米晶为圆柱状,粒径为(225.10±9...

Preparation and in vivo intestinal absorption evaluation of baicalin-berberine complex nanocrystals

[Objective] To investigate the intestinal permeability improvement of baicalin-berberine complex nanocrystals.[Methods] Baicalin-berberine complex nanocrystals were prepared by anti-solvent methodfollowed by high pressure homogenization, and the in situ single-pass intestinal perfusion model was established to evaluate the absorption enhancement of the complex nanocrystals.[Results] Baicalin-berberine complex nanocrystals were prepared and characterized. The baicalin-berberine complex nanocrystals were cylindrical with the particle size at (225.10±9.12) nm, and the Polymer dispersity index was (0.27±0.04). In duodenum, jejunum, ileum and colon, the effective permeability coefficients (P_eff) of baicalin in nanocrystals group were 2.99, 2.00, 2.66 and 5.58 times higher than that of coarse suspension group, separately. The P_eff of berberine in nanocrystals group were 1.25, 1.18, 1.53 and 1.80 times higher than that of coarse suspension group, respectively.[Conclusion] Nanocrystals could improve the oral absorption of baicalin-berberine complex.