Localization of primary cilia in mouse retina |
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Authors: | Yong-Kyu Kim Jin Hyoung Kim Young Suk Yu Hyuk Wan Ko Jeong Hun Kim |
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Affiliation: | 1. Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea;2. Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea;3. Molecular Neurogenetics Laboratory, College of Pharmacy, Dongguk University, Goyang City, Republic of Korea;4. Tumor Microenvironment Research Center, Global Core Research Center, Seoul National University, Seoul, Republic of Korea |
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Abstract: | The primary cilia are considered as “cellular antennae” that sense and interchange information with the extracellular environment. Nearly all mammalian cells have a single primary cilium. In the retina, the outer segment of the photoreceptor is known to be a specialized form of primary cilium, but studies on cilia in other layers of the retina are scarce. In this study, we investigated the expression of primary cilia in the whole thickness of the mouse retina using immunofluorescence with three different ciliary markers: Arl13b, acetylated α-tubulin and adenylyl cyclase III. Our results show positive reactions in the photoreceptor layer, outer plexiform layer and ganglion cell layer, which might suggest the possible presence of primary cilia in these areas, but we could not directly prove the strand-like shape of cilia in those areas. In the outer plexiform layer, all three markers showed intense staining along the neuronal synapses, which suggests that the neuronal processes themselves might share the features of cilia. |
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Keywords: | ADP, adenosine diphosphate AMP, adenosine monophosphate Arl13b, ADP-ribosylation factor-like protein 13B ATP, adenosine triphosphate CC, connecting cilium GCL, ganglion cell layer GST, glutathione-S-transferase GTP, guanosine triphosphate IFT, intraflagellar transport INL, inner nuclear layer IPL, inner plexiform layer IS, inner segment NFL, nerve fiber layer ONL, outer nuclear layer OPL, outer plexiform layer OS, outer segment PBS, phosphate-buffered saline PRL, photoreceptor layer |
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