Anti-thrombosis effect of LRRFIP1 shRNA lentivirus in a mouse model of deep vein thrombosis |
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Authors: | Xiang Yin Siyu WuZiming Wang Yu WangQuanyin Du Aimin Wang |
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Affiliation: | Department of orthopaedics, Institute of surgery research, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China |
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Abstract: | IntroductionDeep vein thrombosis (DVT) is one of the common complications of orthopedic surgery. Low molecular weight heparin (LMWH) is a usually used agent for DVT, but it would increase the risk of bleeding. LRRFIP1 has been shown to play an important role in the formation of thrombosis. Therefore, we investigated the effect of LRRFIP1 shRNA lentivirus on DVT in mice.Materials and MethodsLentiviral Vectors carrying LRRFIP1 shRNA were constructed and transfected into cultured mouse bone marrow cells (BMCs). Male ICR mice were irradiated with a single dose of 9.5 Gy and then were injected with different agents through the tail vein. Stasis venous thrombosis was induced by inferior vena cava (IVC) ligation. Mice were sacrificed on the 1st, 3rd and 7th day post operation and the thrombi were removed, blotted the excess blood on it with filter paper and immediately weighed. P-selectin and d-Dimer were determined by enzyme-linked immunosorbent assay (ELISA).ResultsLRRFIP1 shRNA significantly suppressed the expression of LRRFIP1 in the thrombi. In contrast, low molecular weight heparin (LMWH) and negative shRNA exhibited little effect on the expression of LRRFIP1. LRRFIP1 shRNA, LMWH and negative shRNA inhibited the thrombus formation in vivo significantly. The plasma P-selectin and d-Dimer levels were significantly increased after IVC ligation. LRRFIP1 shRNA significantly decreased the plasma P-selectin and d-Dimer levels. However, LMWH and negative shRNA showed little effects on the levels of plasma P-selectin and d-Dimer.ConclusionLRRFIP1 shRNA might represent a promising prevention strategy for DVT. |
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Keywords: | bone marrow cells, BMCs deep vein thrombosis, DVT enzyme-linked immunosorbent assay, ELISA green fluorescent protein, GFP inferior vena cava, IVC leucine-rich repeat in Flightless-1 interaction protein 1, LRRFIP1 low molecular weight heparin, LMWH pulmonary embolism, PE small interfering RNA, siRNA total hip arthroplasty, THA total knee arthroplasty, TKA |
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