乙型肝炎病毒e抗原阴性慢性乙型肝炎与前C基因及C基因变异关联性临床研究

目的临床观察乙型肝炎病毒e抗原（HBeAg）阴性的慢性乙型肝炎（e-CHB）与前C基因及C基因变异的关联性。方法将慢性乙型肝炎193例进行血清HBeAg、乙型肝炎病毒脱氧核糖核酸（HBV-DNA）水平、前C基因核苷酸1896G→A点突变及C基因核苷酸1762碱基A→T和1764G→A联合突变的检测。结果慢性乙型肝炎193例中,①HBeAg阴性70例（36.27%）,HBeAg阳性123名（63.73%）。②HBeAg阴性前C基因变异率76%,HBeAg阳性前C基因变异率31%,二者比较差异有统计学意义（P〈0.05）;HBeAg阴性C基因变异率51%,HBeAg阳性C基因变异率30%,二者比较差异无统计学意义（P〉0.05）。③HBeAg阳性患者中,前C基因或C基因变异群的HBV-DNA水平低于未变异群,差异比较有统计学意义（P〈0.05）,而HBeAg阴性患者中,前C基因或C基因变异群与非变异群比较,血清HBV-DNA水平差异比较均无统计学意义（P〉0.05）。结论在e-CHB中,前C基因变异与HBeAg转阴具有密切关系,而C基因变异与HBeAg转阴关系不明确;前C基因和C基因变异不能增强病毒毒性,与疾病严重程度可能无关。

Clinical investigation of association of mutations in the core promoter and precore region of HBeAg negative chronic hepatitis B

Objective To investigate the association of mutations in the core promoter and precore region of HBeAg negative chronic hepatitis B.Methods Serum HBeAg,HBC-DNA,mutations in the core promoter and precore region in 193 patients with chronic hepatitis B were detected using enzyme-linked mini-sequence assay kit and HBV core promoter mutation detection kit Results There were 123(63.73%) HBeAg-positive and 70(36.27%) HBeAg-negative patients.HBeAg-negative patients had significantly lower HBV DNA levels compared w...