Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains |
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Authors: | Toshiaki Hino Miho Takabe Rika Suzuki-Migishima Minesuke Yokoyama |
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Institution: | (1) Mitsubishi Kagaku Institute of Life Sciences, Tokyo;(2) Brain Research Institute, Niigata University, 1-757 Asahimachi dori, Chuo-ku, 951-8585 Niigata, Japan |
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Abstract: | Aim Cryopreservation of mouse sperm commonly uses raffinose, which is a trisaccharide, plus 3% skim milk. Because of the present
lack of knowledge of the effectiveness of any other saccharides, we examined the cryoprotective effects of various saccharides
on the viability of mouse sperm from various strains to determine which saccharides are the best cryoprotectants for mouse
sperm.
Methods Sperm from the caudae epididymides of mature C57BL/6J mice were frozen with monosaccharides (fructose, glucose, rhamnose,
xylose), disaccharides (lactose, maltose, sucrose, trehalose) or trisaccharides (melezitose, raffinose) in a range of concentrations
(4–33%). After thawing, the optimal concentration was determined to be the concentration in which there was the highest proportion
of motile sperm. In addition, sperm of inbred and hybrid mice were frozen with the saccharides at the optimal concentrations
and used for in vitro fertilization.
Results The optimal concentration was 12% for the disaccharides and 18% for the trisaccharides. The fertility of all strains, except
C57BL/6J, showed the best cryoprotective effects with maltose, melezitose and raffinose when compared with fresh sperm.
Conclusion Maltose, melezitose and raffinose have the best effects when used as a protectant for cryopreservation of mouse sperm. |
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Keywords: | cryopreservation in vitro fertilization mouse sperm protectant saccharides |
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