金麻杏止咳片快速薄层色谱鉴别与3成分同时定量测定研究

目的 建立简便、快捷的金麻杏止咳片质量控制方法.方法 在5块薄层板上鉴别前胡、黄芩、麻黄、枇杷叶、金银花、苦杏仁和桔梗.采用高效液相色谱法,以乙腈-甲醇-0.1%磷酸(4.5:9:86.5)为流动相,测定样品中盐酸麻黄碱、盐酸伪麻黄碱与苦杏仁苷的含量.结果 通过方法学考察,盐酸麻黄碱进样量在0.0505～0.505 μg(r=0.999997),苦杏仁苷进样量在0.1488～1.488μg(r=0.99996)范围内分别与峰面积呈良好的线性关系.盐酸麻黄碱回收率为100.14%,RSD为1.98%(n=9);苦杏仁苷回收率为97.88%,RSD为1.64%(n=9).结论 所用方法简便、快捷、实用,可用于金麻杏止咳片的质量控制.

Study of Rapid TLC Identification and Simultaneous Quantitative Determination of Three Components in Jinmaxing Zhike Tablets

Objective To establish a simple and rapid method for the quality control of Jinmaxing Zhike Tablets. Methods Peucedani radix, Scutellariae Radix, Ephedrae Eriobotryae Herba, Eriobotryae Folium, Lonicerae Japonicae Flos, Armeniacae Semen Amarum and Platycodonis Radix were identified by TLC at 5 thin-layer chromatographic plates. The content of ephedrine hydrochloride, pseudoephedrine hydrochloride and amygdalin in the sample was determined using a mixture of acetonitrile-methanol- 0. 1% phosphoric acid （4.5：9：86.5） as the mobile phase by HPLC. Results The methodological study showed that a good linear correlation existed in the range 0. 050 5 -0.50 5 ug（ r =0. 999 997）of ephedrine hydrochloride and 0. 148 8 - 1. 488 ug（ r =0. 999 96）of amygdalin. The average recovery of ephedrine hydrochloride and amygdalin was 100. 14% and 97.88% respectively; RSD was 1.98% （n=9） and 1.64% （n = 9） respectively. The negative sample had no interference on TLC identification. Conclusion The method is simple, rapid and us- able,which can be used for the rapid quality control of Jinmaxing Zhike Tablets.