靛玉红大鼠体内吸收药代动力学研究

目的 建立测定大鼠血浆中靛玉红质量浓度的高效液相色谱法,考察靛玉红在大鼠体内的吸收动力学特征.方法 大鼠单剂量口服25mg靛玉红,采用高效液相色谱法测定血浆中靛玉红的质量浓度.结果 靛玉红在大鼠体内分布符合二室模型,主要药物代谢动力学参数分别为峰浓度(Cmax)为(48.63±5.68)ng/mL,达峰时间(Tmax)为(18.76±3.55)h,分布相半衰期(t1/2a)为(38.96±9.51)h,消除相半衰期(t1/2β)为(223.81±154.69)h,0~t药时曲线下面积(AUC0-t)为(2.87±1.33)μg·h/L.结论 所建立的高效液相色谱法灵敏、快速,适用于体内靛玉红的质量浓度测定及代谢动力学研究.

Pharmacokinetic Study of Indirubin Absorption in Vivo in Rats

Objective To establish an HPLC method for detecting the plasma concentration of indirubin in rats and to investigate the pharmacokinetic characteristics of indirubin absorption in vivo. Methods Rats orally took indirubin with single dose of 25 mg. The plasma level of indirubin was determined by HPLC. Results The distribution in vivo of indirubin in rats fit the two-compartment model. The main pharmacokinetic parameters were as follows, Cmax = （48.63 ±5.68） ng/mL; tmax = （18.76 ±3.55） h; t1/2± = （38.96 ± 9.51）h; t1/2a = （223.81 ± 154. 69）h; AUC（0-t） = （2. 87 ± 1.33）μg/（h · L）. Conclusion This established HPLC method is sensitive, rapid and suitable to the concentration detection of indirubin in vivo and the pharmacokinetic study of indirubin.