绞股蓝50%乙醇部位调血脂及抗氧化作用研究

目的研究绞股蓝50%乙醇部位对高脂血症小鼠的调血脂及抗氧化作用。方法采用高脂饲料喂养昆明雄性小鼠,建立高血脂模型,给药30 d,测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)活力,计算脏器指数、低密度脂蛋白胆固醇(LDL-C)、动脉硬化指数(AI)和血清HDL-C/TC比值,并观察肝脏病理切片。结果绞股蓝50%乙醇部位能显著降低高脂血症小鼠TC,TG,LDL-C,MDA的含量和AI值,提高血清SOD,HDL-C/TC比值,HDL-C水平(P<0.05或P<0.01)。除肝指数外(P<0.05),绞股蓝50%乙醇部位对肾指数和脾指数均无显著性影响(P>0.05),但能显著降低高脂血症小鼠的体重(P<0.05)。结论绞股蓝50%乙醇部位能显著降低高脂血症小鼠的TC和MDA,有显著的调血脂及抗氧化作用。

Study on Hypolipidemic and Antioxidation Action of 50% Ethanol Fraction in Gynostemma Pentaphyllum

Objective To study the effects of the 50%ethanol fraction from the extract of Gynostemma pentaphyllum（GP） on lipid-decreasing and antioxidant action in mice.Methods High-fat diet was used to make the hyperlipidemia mice model.The mice were divided into four groups：control,high fat diet（HFD）,positive HFD control and 50%ethanol fraction treatment.The mice were treated with medication once a day for 30 d.Serum levels of TC,TG,HDL-C and malonaldehyde（MDA） were measured.Also,the activity of super oxidant dismutase（SOD） was determined.Results In comparison with HFD control group,the 50%ethanol fraction treatment group significantly reduced TC,TG,LDL-C,MDA and the arteriosclerosis index（AI）,increased SOD,HDL-C/TC and HDL-C（P〈0.05 or P〈0.01）.In addition to the liver coefficient（P〈0.05）,no significant influences were seen in the renal index and spleen index（P〈0.05）.Conclusion The 50%aqueous alcohol fraction in GP can decrease TC and MDA significantly with obvious anti hyperlipidemic and antioxidant action in mice.