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| 反相高效液相色谱法测定心可舒颗粒中葛根素和芦丁含量 | |
| 引用本文： | 阳华,王文渊,蒋小玲.反相高效液相色谱法测定心可舒颗粒中葛根素和芦丁含量[J].中国药业,2012,21(15):37-38. |
| 作者姓名： | 阳华 王文渊 蒋小玲 |
| 作者单位： | 1. 湖南省永州市药品检验所,湖南,永州,425600 2. 湖南永州职业技术学院,湖南,永州,425600 3. 湖南恒伟药业有限公司,湖南,永州,425000 |
| 摘 要： | 目的 建立同时测定心可舒颗粒中主药有效成分葛根素和芦丁含量的方法.方法 采用Symmetry C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-水-乙腈-冰醋酸(28:60:10:2),流速为0.8 mL/min,检测波长为252 nm,柱温为30℃.结果 葛根素质量浓度在2.54～25.40μg/mL的范围内与峰面积呈良好线性关系(r=0.999 8),平均回收率为98.64%,RSD=2.25%(n=6);芦丁质量浓度在1.97～19.70 μg/mL范围内与峰面积线性关系良好,r=0.999 6,平均回收率为100.57%,RSD=2.53%(n=6).结论 该法准确、简便,重现性好,可用于心可舒颗粒制剂的质量控制. |
| 关 键 词： | 心可舒颗粒 葛根素 芦丁 高效液相色谱法 |
Determination of Puerarin and Rutinum in Xinkeshu Granules by HPLC |
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| Yang Hua , Wang Wenyuan , Jiang Xiaoling.Determination of Puerarin and Rutinum in Xinkeshu Granules by HPLC[J].China Pharmaceuticals,2012,21(15):37-38. | |
| Authors: | Yang Hua Wang Wenyuan Jiang Xiaoling |
| Institution: | 1.Yongzhou Municipal Institute for Drug Control,Yongzhou,Hunan,China 425606;2.Yongzhou Vocational Technical College,Yongzhou,Hunan,China 425600;3.Hunan Hengwei Pharmaceutical Co.,Ltd.,Yongzhou,Hunan,China 425000) |
| Abstract: | Objective To establish a HPLC method for the determination of puerarin and rutinum in Xinkeshu Granules.Methods The analytical column was the Symmetry C18 column（250 mm×4.6 mm,5 μm） with methanol-water-acetonitrile-glacial acetic acid（28：60：10：2） as the mobile phase.The flow rate was 0.8 mL/min,the detection wavelength was 252 nm and the column temperature was 30 ℃.Results The linear range for puerarin and rutinum were 2.54-25.4 μg/mL（r=0.999 8） and 1.97-19.7 μg/mL（r=0.999 6）,respectively.The average recovery rates were 98.6%,RSD=2.23%（n=6） for puerarin and 100.5%,RSD=2.08%（n=6） for rutinum.Conclusion This method is simple,accurate,better reproducible,and suitable to be used in the quality control of Xinkeshu Granules. |
| Keywords: | Xinkeshu Granules puerarin rutinum HPLC |
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