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Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis |
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| Authors: | T. Yokoyama N. Sugimoto K. Taniguchi S. Komoto T. Yuno K. Ohta H. Hashimoto A. Seno A. Ashida M. Fujieda S. Nishio K. Ueno M. Shimizu A. Yachie |
| Affiliation: | 1. Department of Paediatrics, Ishikawa, Japan;2. Department of Physiology, Kanazawa University Graduate School of Medical Science, Ishikawa, Japan;3. Department of Virology and Parasitology, Fujita Health University School of Medicine, Aichi, Japan;4. Department of Medical Laboratory, Kanazawa Red Cross Hospital, Ishikawa, Japan;5. Department of Paediatrics, National Health Organization, Kanazawa Medical Centre, Ishikawa, Japan;6. Department of Paediatrics, Kanazawa Red Cross Hospital, Ishikawa, Japan;7. Department of Paediatrics, Kanazawa City Hospital, Ishikawa, Japan;8. Department of Paediatrics, Osaka Medical College, Osaka, Japan;9. Department of Paediatrics, Kochi Medical School, Kochi, Japan |
| Abstract: | This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis. |
| Keywords: | Immunohistochemistry inclusion-bearing cells rotavirus gastroenteritis RT-PCR urinary sediment cells |
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