木犀草素对慢性肾衰竭大鼠的保护作用

目的:探讨木犀草素对慢性肾衰竭大鼠的保护作用.方法:采用腺嘌呤法对大鼠进行灌胃,连续30 d,建立大鼠慢性肾衰竭模型,对照组灌胃生理盐水.造模成功后将18只大鼠随机分为模型组、木犀草素高剂量组、木犀草素低剂量组,给予相应药物治疗15d.观察大鼠体态、毛发颜色及活动状况,ELISA法检测大鼠血清IL-6、TNF-α、BU...

Protective Effect of Luteolin on Rats with Chronic Renal Failure

Objective:To investigate the protective effect of luteolin on rats with chronic renal failure.Methods:Chronic renal failure model was established by intragastric administration of adenine for 30 days,and normal saline was given to the control group.After successful modeling,18 rats were randomly divided into model group,luteolin high-dose group and luteolin low-dose group,and were treated with corresponding drugs for 15 days.The body shape,hair color and activity of rats were observed.The serum levels of IL-6,TNF-α,BUN and SCr were detected by ELISA.The pathological changes of kidney tissue were observed by HE staining.The protein expression levels of TLR4,p-p65 and P-IκB in kidney tissue were detected by Western blotting.Results:The rats in the control group had shiny hair,round body,agile movements,normal kidney volume,normal dark red color,and normal serum test indicators.Compared with the control group,the rats in the model group have rough and dull hair,bald hair,and slow movement.The levels of serum IL-6,TNF-α,BUN,SCr and UA in the model group were significantly increased(P<0.05).The kidney tissue is enlarged in volume,pale and bloodless,with a large number of inflammatory infiltrations,interstitial fibrosis,and severe dilation of the renal capsule.The expression levels of TLR4,p-p65,and p-IκB proteins in the kidney tissue were significantly increased(P<0.05).Compared with the model group,the rats in the luteolin high-dose group had normal body shape,dull hair,no depilation,less inflammatory infiltration in kidney tissue,mild dilatation of renal capsule cavity,mild degree of interstitial fibrosis,blood color in kidney tissue,the levels of serum IL-6,TNF-α,BUN,SCr and UA were significantly decreased(P<0.05),and the expression levels of TLR4,p-p65 and p-IκB in kidney tissue were significantly decreased(P<0.05).Conclusion:High-dose luteolin can improve renal function damage and reduce inflammation in rats with chronic renal failure.The mechanism may be inhibition of TLR4/NF-κB signaling pathway.