HPLC法测定多维铁口服溶液中维生素B_1、维生素B_2的含量

目的:建立以高效液相色谱法测定多维铁口服溶液中维生素B1、维生素B2含量的方法。方法:色谱柱为C18,流动相为离子对溶液(含1·5mmol·L-1戊烷磺酸钠和3·4mmol·L-1庚烷磺酸钠的0·24%三乙胺溶液,用冰醋酸调节pH值至3·0)-甲醇(80:20),检测波长为254nm,流速为1·0mL·min-1,进样量为10μL。结果:维生素B1、维生素B2检测浓度的线性范围分别为4·81～24·6(r=0·9991)、6·22～31·7μg·mL-1(r=0·9998);平均回收率分别为103·3%(RSD=0·5%)、99·5%(RSD=0·7%)。结论:本方法简便准确、灵敏度高、结果可靠,可用于该制剂中维生素B1、维生素B2的含量测定。

Determination of Vit B1 and Vit B2 in Duoweitie Oral Liquid by HPLC

OBJECTIVE： To determine the contents of yitamin B1 and vitamin B2 in duoweitie oral liquid by HPLC. METHODS： Samples were separated on C18, the mobile phase consisted of ion pair solution（0.24% of triethylamine solution containing 1.5 mmol· L^-1 of sodium pentanesulfonate and 3.4 mmol·L^-1 of sodium heptanesulfonate with pH adjusted to 3.0 by glacial acetic acid） -methanol （80 ： 20） at a flow rate of 1.0 mL· min^-1. The detection wavelength was set at 254 nm and the sample size was 10 μL. RESUI.TS： The linear ranges of vitamin B, and vitamin B2 were 4.81-24.6（r =0.999 1） and 6.22-31.7μg· mL^-1（r = 0.999 8）, respectively, and the average recovery rates were 103.3% （RSD = 0.5% ） and 99.5% （RSD = 0.7% ）, respectively. CONCLUSION： This method is simple, accurate, sensitive and reliable, and suitable for the determination of vitamin B1 and vitamin B2 in duoweitie oral liquid.