| 紫草素通过PI3K/Akt通路促进人乳腺癌MCF-7细胞自噬 |
| |
| 引用本文： | 陈菊英,刘朝纯,曾智,黄文东,杨永飞,朱邦豪. 紫草素通过PI3K/Akt通路促进人乳腺癌MCF-7细胞自噬[J]. 中国药理学通报, 2013, 29(2): 194-198 |
| |
| 作者姓名： | 陈菊英 刘朝纯 曾智 黄文东 杨永飞 朱邦豪 |
| |
| 作者单位： | 中山大学中山医学院药理学教研室,广东,广州,510080 |
| |
| 基金项目： | 粤港关键领域重点突破项目(No 2007A032702001) |
| |
| 摘 要： | 目的研究紫草素(Shikonin)对人乳腺癌MCF-7细胞自噬的影响及其作用机制。方法 CCK-8法检测紫草素处理人乳腺癌MCF-7细胞24、48 h细胞的存活率,Western blot方法检测紫草素处理24 h和48 h时LC3、p62、PI3K、Akt、p-PI3K、p-Akt蛋白水平。结果 1μmol.L-1紫草素处理细胞48 h以及2.5、5μmol.L-1紫草素处理MCF-7细胞24 h和48 h时,MCF-7细胞的活力受到明显抑制,LC3-Ⅱ/LC3-Ⅰ值增加,p62表达减少,总PI3K、Akt、p-PI3K、p-Akt均减少。结论紫草素促进乳腺癌MCF-7细胞自噬,其作用机制可能与PI3K/Akt通路受到抑制有关。
|
| 关 键 词： | 紫草素 MCF-7细胞 自噬 LC3 p62 PI3K Akt p-PI3K p-Akt |
Shikonin promotes autophagy of MCF-7 human breast cancer cells through PI3K/Akt pathway |
| |
| CHEN Ju-ying , LIU Zhao-chun , ZENG Zhi , HUANG Wen-dong , YANG Yong-fei , ZHU Bang-hao. Shikonin promotes autophagy of MCF-7 human breast cancer cells through PI3K/Akt pathway[J]. Chinese Pharmacological Bulletin, 2013, 29(2): 194-198 |
| |
| Authors: | CHEN Ju-ying LIU Zhao-chun ZENG Zhi HUANG Wen-dong YANG Yong-fei ZHU Bang-hao |
| |
| Affiliation: | (Dept of Pharmacology,Zhongshan School of Medicine,Sun Yat-Sen University,Guangzhou 510080,China) |
| |
| Abstract: | Aim To explore the effect of shikonin on the autophagy of MCF-7 human breast cancer cells and its mechanism.Methods The cell viabilities were examined by CCK-8 assay.And the expressions of LC3,p62,PI3K,Akt,p-PI3K,p-Akt were tested by western blot assay.Results The cell viabilities of MCF-7 cells were significantly inhibited,the values of LC3-Ⅱ/LC3-Ⅰ were elevated,and the protein levels of p62,PI3K,Akt,p-PI3K,p-Akt were all decreased after treated with 1 μmol·L-1 shikonin 48 h and 2.5,5 μmol·L-1 shikonin 24 h,48 h.Conclusions Shikonin can promote the autophagy of MCF-7 cells,and the possible mechanism is related to the PI3K/Akt signaling pathway. |
| |
| Keywords: | shikonin MCF-7 cells autophagy LC3 p62 PI3K Akt p-PI3K p-Akt |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
