| 猪源致病性沙门氏菌四环素耐药基因tetC的PCR扩增及序列分析 |
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| 引用本文： | 代敏,王红宁,吴琦,李春,于新芬. 猪源致病性沙门氏菌四环素耐药基因tetC的PCR扩增及序列分析[J]. 中国抗生素杂志, 2004, 29(7): 415-417,434 |
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| 作者姓名： | 代敏 王红宁 吴琦 李春 于新芬 |
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| 作者单位： | 1. 四川农业大学动物科技学院,雅安,625014;绵阳师范学院分子生物学与生物制药重点实验室,绵阳,62100
2. 四川农业大学动物科技学院,雅安,625014 |
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| 基金项目： | 国家“十五”国家科技攻关课题资助 (编号 :2 0 0 2 BA51 4 A- 1 7- 1 1 )。 |
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| 摘 要： | 本研究对16株猪致病性沙门氏菌的四环素耐药性进行了检测，结果表明，沙门氏菌对四环素类抗生素产生了广泛的耐药性，耐药率达100％。对四环素耐药基因tetC进行PCR扩增，结果在12株沙门氏菌的质粒上扩增出特异性产物，与药敏试验结果阳性符合率75％，具有较高的检出率；序列分析结果表明tetC的核苷酸同源率较高，达97．7％～98．7％，建立tetC基因的PCR检测技术，为沙门氏菌对四环素耐药性的分子流行病学监测提供了有效的途径。
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| 关 键 词： | 沙门氏菌 四环素耐药基因 tetC PCR 序列分析 |
| 文章编号： | 1001-8689(2004)07-0415-03 |
PCR amplification and nucleotide sequences analysis of tetC gene of pathogenic Salmonella from swine |
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| Dai Min ,,Wang Hong-ning,Wu Qi,Li Chun and Yu Xin-fen. PCR amplification and nucleotide sequences analysis of tetC gene of pathogenic Salmonella from swine[J]. Chinese Journal of Antibiotics, 2004, 29(7): 415-417,434 |
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| Authors: | Dai Min Wang Hong-ning Wu Qi Li Chun Yu Xin-fen |
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| Affiliation: | Dai Min 1,2,Wang Hong-ning1,Wu Qi1,Li Chun1 and Yu Xin-fen1 |
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| Abstract: | The detection of tetracyclines-resistance of 16 Salmonella isolates showed the resistance rate was 100%. The tetC gene of 16 Salmonella strains was amplified by polymerase chain reaction. Twelve strains with this gene were located on plasmids. Compared to the results of the drug-susceptibility tests, the method of PCR showed 75% concordance in positive rate. The method of using PCR to detect tetC gene was established, and it could be applied to the molecular epidemiology surveillance of tetracyclines-resistance. |
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| Keywords: | Salmonella Tetracycline-resistant gene tetC PCR Nucleotide sequences analysis |
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