羟基红花黄色素A对大鼠脑缺血损伤的神经保护作用

分别用电凝法烧灼凝结大鼠大脑中动脉和四动脉闭塞法建立大鼠局灶性永久性脑缺血模型和全脑缺血-再灌注模型。考察羟基红花黄色素A的低、中、高剂量组(1、2和4mg/kg)、尼莫地平组(2mg/kg)和生理盐水组的神经保护作用及对大鼠行为缺陷的改善作用。结果表明,对于局灶性永久性脑缺血大鼠,羟基红花黄色素A各剂量组的脑坏死区重量均比生理盐水组明显降低,高、中剂量组还可明显改善大鼠的行为学缺陷;尼莫地平组与生理盐水组无显著性差异。对于全脑缺血-再灌注大鼠,羟基红花黄色素A各剂量组与生理盐水组相比,均能延长大鼠缺血后脑电图消失时间,明显缩短灌注后脑电图恢复时间和翻正反射恢复时间,并明显改善脑水肿情况。

Neuroprotective Effect of Hydroxysafflor Yellow A on Cerebral Ischemia Injuries in Rats

Focal permanent cerebral ischemia model and global cerebral ischemia-reperfusion model were establishedby electrocoagulation of the middle cerebral artery and four arteries occlusion in rats method, respectively. The neuroprotectiveeffect as well as the improvement of neurological deficits of rats of the low, middle and high dosage groups of hydroxysaffloryellow A(1, 2 and 4mg/kg), nimodipine group(2mg/kg) and saline group was investigated. The results showed that threehydroxysafflor yellow A groups could significantly decrease the weight of cerebral infarction area in focal permanent cerebralischemia rats compared with saline group. High and middle dosage groups could significantly improve the neurologicaldeficits as well. Nimodipine group had no significant difference with saline group. The three groups of hydroxysafflor yellowA significantly prolonged electroencephalogram disappearing time, shortened righting reflex and electroencephalogramrecovering time and improve the cerebral eddema in global cerebral ischemia-reperfusion rats compared with saline group.