| Abstract: | Membrane-bound HL-A antigens and μ-chain determinants were studied on normal and leukemic lymphocytes and on a tissue culture cell line derived from a Burkitt's tumor, using membrane immunofluorescence on living cells. A redistribution of both antigens at the cell surface occurred when cells labeled in the cold by conjugated antisera were incubated at 37 °C. Double labeling experiments showed that HL-A antigens, either of the first or the second sublocus, were unaffected by the redistribution of IgM determinants and vice versa. This result strongly suggests molecular independence of these two systems. When redistribution was induced with a mixture of conjugated sera to HL-A and Ig, the caps showing each of the two specificities were located at the same pole of the cells. |
