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| 细胞生理条件下壳聚糖微胶囊包埋肝细胞 | |
| 引用本文： | 朱建航,张宝,严喜鸾,劳学军,Yu Hanry. 细胞生理条件下壳聚糖微胶囊包埋肝细胞[J]. 生物医学工程学杂志, 2006, 23(5): 1070-1074 |
| 作者姓名： | 朱建航 张宝 严喜鸾 劳学军 Yu Hanry |
| 作者单位： | 1. 南昌大学,化学工程系,南昌大学,食品科学教育部重点实验室,南昌,330029;新加坡国立大学,医学院,生理学系,新加坡,117597 2. 江西农业大学,生物工程系,南昌,330045 3. 南昌大学,化学工程系,南昌大学,食品科学教育部重点实验室,南昌,330029 4. 新加坡国立大学,医学院,生理学系,新加坡,117597;暨南大学,医学院,广州,510220 5. 新加坡国立大学,医学院,生理学系,新加坡,117597 |
| 摘 要： | 从乙酰化率15．3％的市售壳聚糖（Fifteen N-acetylated chitosan，FNC）出发制备酰化率50％的壳聚糖衍生物（Half N-acetylated chitosan，HNC），探讨其在细胞生理条件下与异丁烯酸（Methacrylic acid，MAA）-羟乙基异丁烯（Hydroxyethyl methacrylate，HEMA）-甲基丙烯酸甲酯（Methyl methacrylate，MMA）三元共聚物（MAA-HEMA-MMA）制备微胶囊包埋肝细胞的可行性。微囊化细胞实验结果表明，与体外表面培养实验相比，生理条件下基于HNC构建的三维正电微环境有利于支持肝细胞的体外功能，且制备的微胶囊在培养过程中具有较好的渗透性及结构稳定性。 |
| 关 键 词： | 壳聚糖 微胶囊 肝细胞 细胞功能 |
| 收稿时间： | 2004-12-16 |
| 修稿时间： | 2004-12-162005-04-27 |
Encapsulating Hepatocytes with Chitosan in Physiological Conditions |
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| Zhu Jianhang,Zhang Bao,Yan Xiluan,Lao Xuejun,Yu Hanry. Encapsulating Hepatocytes with Chitosan in Physiological Conditions[J]. Journal of biomedical engineering, 2006, 23(5): 1070-1074 | |
| Authors: | Zhu Jianhang Zhang Bao Yan Xiluan Lao Xuejun Yu Hanry |
| Affiliation: | Department of Chemical Engineering, Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330029, China. envzjh@ncu.edu.cn |
| Abstract: | Prepared from 15.3% N-acetylated chitosan(FNC),half N-acetylated chitosan(HNC) possesses a good solubility in a weak basic solution,guaranteeing the formation of microcapsules by the coacervating reaction between HNC and methacrylic acid(MAA)-hydroxyethyl methacrylate(HEMA)-methyl methacrylate(MMA)(MAA-HEMA-MMA) terpolymer under physiological conditions.When hepatocytes were encapsulated in such 3-dimensional microenvironment,as compared to monolayer culture,cell functions,including P450 activity,urea production and albumin release,were well supported.The prepared microcapsules have good mechanical stability and permeability. |
| Keywords: | Half N-acetylated chitosan(HNC) Microcapsule Hepatocyte Cell functions |
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