Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae |
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Authors: | Chen Chang-Lin Pan Tzu-Ying Kan Shu-Chen Kuan Yi-Chia Hong Lian-Yu Chiu Kun-Ruei Sheu Ching-Sen Yang Jui-Sen Hsu Wen-Hwei Hu Hui-Yu |
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Affiliation: | a Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan b Vedan Enterprise Co., Shalu, Taichung 433, Taiwan c Department of Life Science, National Taiwan Ocean University, Keelung 202, Taiwan d Department of Food and Nutrition, Hungkuang University, Shalu, Taichung 433, Taiwan |
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Abstract: | P1201 is a lytic corynephage of Corynebacterium glutamicum NCHU 87078. Its genome consists of a linear double-stranded DNA molecule of 70,579 base pairs, with 3′-protruding cohesive ends of ten nucleotides. We have identified 69 putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and RNR α subunit genes) that are interrupted by an intein. Protein-splicing activities of these inteins were demonstrated in Escherichia coli. Three structural proteins including major capsid and major tail proteins were separated by SDS-PAGE and identified by both LC-MS-MS and N-terminal sequence analyses. Bioinformatics analysis indicated that only about 8.7% of its putative gene products shared substantial protein sequence similarity with the lytic corynephage BFK20 from Brevibacterium flavum, the only corynephage whose genome had been sequenced to date, revealing that the P1201 genome is distinct from BFK20. The mosaic-like genome of P1201 indicates extensive horizontal gene transfer among P1201, Gordonia terrae phage GTE5, mycobacteriophages, and several regions of Corynebacterium spp. genomes. |
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Keywords: | Corynebacterium glutamicum Corynephage Lytic phage Complete genome sequence Sequence analysis |
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