CagA基因阳性Hp培养滤液对人胃粘膜上皮细胞系DNA损伤作用

目的 研究 CagA阳性幽门螺杆菌（Hp）培养滤液对人胃粘膜上皮细胞（GES－1）DNA的损伤作用。方法 制备Hp培养滤液，PCR鉴定CagA基因。采用单细胞微凝胶电泳观察Hp（CagA＋）培养滤液对GES－1细胞DNA的损伤作用。结果 Hp（CagA＋）培养滤流可使GES－1细胞形成彗星现象。结论 Hp（CagA＋）培养滤液可以导致GES－1细胞的DNA损伤。DNA损伤可能是CagA诱导GES－1细胞恶性转化的机制之一。

Effects of broth culture filtrates of CagA gene positive Helicobacter pylori on DNA damage of human gastric epithelial cell line

Objective To study the effects of broth culture filtrates of CagA gene positive Helicobacter pylori on DNA damage of human gastric epithelial cells line. Methods Broth culture filtrates of Helicobacter pylori was prepared. CagA gene were identified by PCR. GES-I cells were treated with CagA gene positive broth culture filtrates of Helicobacter pylori. Single cell microgel electrophoresis were used to observe the damage of GES-I cell DNA caused by the broth culture filtrates. Results HP(CagA + ) culture filtrates may cause comet phenomenon of GES-I cells. Con- clusion DNA damage can be induced by borth culture filtrates of CagA gene positive Helicobacter pylori in human gastric epithelial cells GES-I line. DNA damage may be one of the mechanism of malignant transformation induced by broth culture filtrates of CagA gene positive Helicobacter py- lori in human gastric epithelial cells.