标本溶血对常规生化检验结果的影响

目的了解临床标本溶血对丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆固醇(TC)等11项常规生化检验结果的影响,探讨影响因素并在此基础上提出相应对策。方法抽取100例体检者空腹血液标本5mL,分别置于2支试管中,每支2.5mL,其中1支试管中的标本采用人工方法使其溶血,检测正常标本和溶血标本血清中ALT、AST、三酰甘油(TG)、尿素(UREA)、肌酐(Cr)、葡萄糖(GLU)、尿酸(UA)等11项临床常规生化检验指标的含量并进行比较。结果溶血标本血清ALT、AST、总蛋白、清蛋白测定结果明显高于正常标本血清,差异有统计学意义(P<0.05),溶血后总胆红素、Cr、TC、TG及GLU较溶血前均有明显降低,差异有统计学意义(P<0.05),溶血对UREA、UA测定结果影响较小。结论标本溶血对一些常规生化检验项目有明显的干扰作用,临床检验工作中应采取一切可能的措施避免标本溶血的发生。

Influence of hemolysis on biochemical detection

Objective To investigate the influence of sample hemolysis on the detection of biochemical indica- tors. Methods Blood samples of 100 subjects undergoing examination of health screening were taken, 5 mL each one, and each sample was placed in two test tubes respectively, 2.5 mL for each tube. Artificial methods were used to make one of the paired samples hemolysis, and all samples were tested for alanine aminotransferase （ALT）, as- partate aminotransferase （AST）, triglyeride （TG）, UREA, creatinine （Cr）, glucose （GLU） and uric acid （UA） and so on, and the results were compared. Results The contents of serum ALT, AST, total protein, albumin in he- molytic samples were significantly higher than those in normal samples （P~0.05）. The contents of serum total bili- rubin, CREA, total cholesterol, TG and GLU in hemolytic samples were significantly lower than those in normal samples （P~0.05）. Levels of UREA and CREA changed little between hemolytic and normal samples. Conclusion There might be with obvious interference of hemolysis on biochemical test items. All possible measures should be taken to prevent samples hemolysis.