骨髓间充质干细胞对大鼠急性一氧化碳中毒脑损伤的作用及机制研究

 目的  观察骨髓间充质干细胞(MSCs)对大鼠急性一氧化碳（CO）中毒脑损伤的作用并探讨其机制。  方法 体外培养MSCs并标记，制备MSCs悬液;100～150mL/kg腹腔注射CO建立动物模型，随后将标记5-溴脱氧尿嘧啶(Brdu)的MSCs经颈内动脉移植入脑损伤的大鼠体内;采用Morris水迷宫实验检测大鼠神经系统功能;采用免疫组化、尼氏染色方法检测海马损伤区细胞变化。  结果 MSCs在脑内存活并减少神经细胞凋亡，促进神经细胞结构和功能恢复。MSCs移植1、4周后，MSCs组大鼠水迷宫实验显示空间学习能力有所提高，脑组织损伤明显改善(P＜0.05)。 结论 MSCs移植治疗大鼠CO中毒后的脑损伤具有较好的疗效。

Mechanism of mesenchymal stem cells in treatment of brain damage caused by carbon monoxide poisoning (COP) in rats

Objective To investigate the effect and mechanism of mesenchymal stem cells(MSCs) transplanted for brain damage induced by carbon monoxide poisoning(COP) in rats.Methods MSCs were isolated from the bone marrow of Wistar rats,cultured and generated in vitro and then made into a suspension.The rat carbon monoxide poisoning models were established by peritoneal injection of 100-150 mL/kg CO.MSCs marked with Bromodeoxyuridine(Brdu) were transferred into rats through the internal carotid artery.The Morris water ...