| 红细胞带Ⅲ蛋白C1肽链的纯化及其功能的初步研究 |
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| 引用本文： | 傅国辉,姜晓姝,王孝铭,滨奇直孝. 红细胞带Ⅲ蛋白C1肽链的纯化及其功能的初步研究[J]. 中国病理生理杂志, 1999, 15(3) |
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| 作者姓名： | 傅国辉 姜晓姝 王孝铭 滨奇直孝 |
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| 作者单位： | 1. 哈尔滨医科大学病理生理教研室,哈尔滨,150086
2. 日本国九州大学医学部临床检查医学讲座 |
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| 摘 要： | 目的：研究红细胞带Ⅲ蛋白Ｃ１肽链（带Ⅲ蛋白Ａｌａ８９３－Ｖａ１９１１）的特性及其功能。方法：以１００ｍｍｏｌ／ＬＮａＯＨ预处理红细胞膜血影，再用胰蛋白酶消化，采用高效液相色谱（ＨＰＬＣ）及氨基酸测序仪分析检定了从红细胞血影中释放的多肽，并纯化了带Ⅲ蛋白Ｃ１肽链，以不同浓度的Ｃ１肽链与新鲜的红细胞血影进行温浴。结果：Ｃ１肽链与红细胞内的新蛋白酶活性有关。
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| 关 键 词： | 带3蛋白质；肽类；红细胞 |
Purification and characterization of C1 peptide released from band 3 protein |
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| FU Guo-Hui,JIANG Xiao-Shu,WANG Xiao-Ming,Naotaka Hamasaki. Purification and characterization of C1 peptide released from band 3 protein[J]. Chinese Journal of Pathophysiology, 1999, 15(3) |
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| Authors: | FU Guo-Hui JIANG Xiao-Shu WANG Xiao-Ming Naotaka Hamasaki |
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| Abstract: | AIM: In order to study the properties of C1 peptide(band 3 Ala893-Va1911).METHODS:The human erythrocyte white ghost were pre-treated by 100 mmol/L NaOH and then digested by trypsin. Using the HPLC and amino acid sequencer, we identified the peptides that released from white ghost, and purified the C1 peptides(band 3 Ala893-Va1911). The C1 peptides were used to incubate with fresh white ghosts.RESULTS:C1 peptides related with a novel protease activity. |
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| Keywords: | Band 3 protein Peptides Erythrocytes |
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