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| 陶瓷化骨对人牙囊细胞分化的影响 | |
| 引用本文： | 常秀梅,刘宏伟,陈坤,金岩.陶瓷化骨对人牙囊细胞分化的影响[J].临床口腔医学杂志,2009,25(6). |
| 作者姓名： | 常秀梅 刘宏伟 陈坤 金岩 |
| 作者单位： | 1. 广东医学院附属医院口腔科,广东,湛江,524001 2. 上海同济大学口腔医学院,上海,200072 3. 第四军医大学口腔医学院病理科·组织工程中心,陕西,西安,710032 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的:培养人牙囊细胞(DFCs),观察陶瓷化骨(CBB)对人牙囊细胞生长和分化的影响.方法:体外分离胚胎期人DFCs,将第三代细胞以CBB为载体立体培养,以平面接种为对照组,用矿化液持续诱导,利用光镜观察细胞生长状态、免疫组织化学观察细胞矿化基质牙骨质附着蛋白(CAP,)及骨涎蛋白抗体(BSP)分泌、Vokassa染色观察矿化情况,了解CBB对DFCs生物学行为的影响.结果:体外连续诱导28 d,实验组光镜下可见DFCs与CBB相容性好,材料周边细胞生长密集,并有向BBC趋化迁移现象,附近细胞聚集,形成Vokassa染色阳性的矿化结节,免疫组织化学染色BSP,CAP阳性着色,部分细胞强阳性着色.对照组细胞复层生长,未形成矿化结节,免疫组织化学染色BSP,CAP阴性着色.结论:体外培养条件下,CBB可诱导人DFCs向成骨和成牙骨质细胞分化. |
| 关 键 词： | 陶瓷化骨 牙囊细胞 诱导 分化 |
Ceramic bovine bone affects cellar behavior of purified human dental follicle cells. |
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| CHANG Xiu-mei,LIU Hong-wei,CHEN Kun,JIN Yan.Ceramic bovine bone affects cellar behavior of purified human dental follicle cells.[J].Journal of Clinical Stomatology,2009,25(6). | |
| Authors: | CHANG Xiu-mei LIU Hong-wei CHEN Kun JIN Yan |
| Institution: | CHANG Xiu-mei1,LIU Hongwei2,CHEN Kun1,JIN Yan3. 1.Department of Stomatology,The Affiliated Hospital of Guangdong Medical College,Guangdong Zhanjiang 524001,China,2.School of Stomatology,Tongji University,Shanghai 200072,3.Research , Develepment Center for Tissue Engineering,Department of Oral Histology , Pathology,School of Stomatology,The Fourth Military Medical University,Shanxi Xi'an 710032 |
| Abstract: | Objective:To investigate the Effect of ceramic bovine bone on behave of purified human dental follicle cells. Method:Human dental follicle cells was cultured in vitro.The subsequent cell passaging were conducted in conditioned medium containing dexamethasone,beta -sodium glycerophosphate and ascorbid acid.with the human dental follicle cells in curture then divided into BBC (in which BBC was added in T -flask)and Hdfc group (without BBC). Migration was observed under inverted phase microscope and differenti... |
| Keywords: | ceramic bovine bone dental follicle cell behavior |
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