丁咯地尔对慢性脑缺血大鼠海马CA1区星形胶质细胞及认知障碍的影响

目的研究丁咯地尔对慢性脑缺血大鼠海马CA1区星形胶质细胞和认知障碍的影响。方法采用双侧颈总动脉永久性结扎制备慢性脑缺血模型，治疗组大鼠给与丁咯地尔灌胃，免疫组化法多克隆抗血清GFAP标记海马CA1区星形细胞，用Y-型迷宫测定大鼠的认知功能变化。实验研究为持久性2VO2个月。结果慢性脑缺血2个月后大鼠海马CA1区星形胶质细胞大量增生肥大，认知能力明显下降，丁咯地尔治疗后，星形胶质细胞的活动明显减少，认知功能明显提高。结论丁咯地尔能抑制慢性脑缺血大鼠海马CA1区星形胶质细胞反应，改善其认知功能障碍。

Effects of buflomedil on astrocytes at CA1 of hippocampus and cognitive impairment in rats with chronic cerebral ischemia

Objective To investigate the effects of builomedil on astrocytes at CA1 of hippocampus and cognitive impairment in aging rats with chronic cerebral ischemia. Methods Chronic cerebral ischemia was performed in aging rats by permanent bilateral common carotid artery occlusion method, the treatment group was filled buflomedil. Astrocytes were observed with immunohistochemistry for GFAP. The Y- type maze was used to test the cognitive function of rats. The investigation duration lasted two months after the ligation. Results There were obvious proliferation and hypertrophy of astrocytes at CA1 of hippocampus after two months of chronic cerebral ischemia. The cognitive function of rats was significantly reduced. In buflomedil- treated rats, the expression of proliferation and hypertrophy of astrocytes decreased significantly reduced. The cognitive function of rats was distinctly improved. Conclusion Buflomedil could suppress the activation of astrocytes at CA1 of hippocampus, and improve the cognitive function of aging rats with chronic cerebral ischemia.