清肝灵对a-萘异硫氰酸酯所致急性肝损害保护作用的实验研究

目的:观察中药清肝灵煎剂对α-萘异硫氰酸酯(ANIT)急性肝损伤的保护作用及其机制。方法:将SD大鼠40只随机分为5组,分别为正常对照组,模型组,茵栀黄组,清肝灵低、高剂量组。除正常对照组外,其它4组大鼠均用ANIT(100mg/kg)灌胃造成急性肝损伤。各组动物于ANIT致毒后分别给予相应药物或生理盐水处理。检测各组动物ANIT中毒后24、48、72h的肝功能以及72h血清内毒素(ET)、一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)的浓度,同时观察各组大鼠肝组织病理改变。结果:清肝灵低、高剂量组的肝功能、病理改变情况明显优于模型组、菌栀黄组,同时清肝灵低、高剂量组的ET、NO和TNF-α的含量均明显低于模型组、菌栀黄组(P<0.01或P<0.05)。结论:清肝灵对ANIT所致大鼠的急性肝损伤有较好的保护作用,其作用可能与减轻内毒素血症,抑制NO、TNF-α等诱导的炎症反应有关。

Experimental study of protective effects of Qingganling apozem on acute hepatic injury induced by alpha-naphthylisothiocyanate in rats

Objective: To investigate the protective effect of Qingganling (QGL) apozem on experimental acute hepatic injury of rats induced by Alpha-naphthylisothiocyanate (ANIT) and explore the mechanism of the effect. Methods: Forty Sprague-Dawley rats were randomly divided into five groups: the normal group, the model group, the control group of Yinzhihuang, the treatment group with low dosage of QGL and the treatment group with high dosage of QGL. All experimental rats except those in the normal group were given a single dose of ANIT (100mg/kg) via gavage to make the acute hepatic injury model, and then all rats were respectively treated with corresponsive drugs after given ANIT. The serum biochemical indices including alanine aminotransferase (ALT) and total bilirubin (TBI) of rats were monitored at 24 h, 48 h and 72 h after the rats were given ANIT. In the end ,blood samples were collected for the detection of the serum endotoxin(ET), nitric oxide(NO), and tumor necrosis factor alpha(TNF-α). The histological changes were observed by light microscope. Results: Compared with the normal group, the levels of serum ALT and TBI in the model group markedly increaced after ANIT ig and reached a peak in 48 hours, and the concentrations of ET, NO, and TNF-α significantly increased too (P< 0.01). In comparison with the model group, the levels of ALT, TBI, ET, NO as well as TNF-α of the rats in the other three groups were lower (P<0.01). Moreover, the changes of all the above indices in the treatment groups with different dosages of QGL were slighter than those in the control group of Yinzhihuang. In all the experimental groups,the most severe liver histological changes were found in the model group, showing obvious cell degeneration and necrosis of hepatocytes and epithelial cells of bile ducts accompanied by obvious inflammatory cell infiltration. These pathological changes in the contral group of yinzhihuang were slighter than those in the model group, but severer than those in the treatmnet groups with different dosages of QGL. Conclusion: The experimental results demonstrate that QGL has good therapeutic effect on acute liver injury of rats induced by ANIT. The hepatoprotective effect is move potent than that of YZH. The possible mechanism of the hepatoprotective effect of QGL may be through the inhibition of inflammation by decreasing the ET, NO and TNF-α.