肝癌与肝炎患者肝内人纤维介素的表达及意义

目的 探讨轻度、中度、重度慢性乙型肝炎及肝癌患者肝组织内人纤维介素（又称人纤维蛋白原样2凝血酶原酶,human fibrinogen-like protein 2 prothrombnase,h Fgl 2）的表达及其与患者血清转氨酶（ALT、AST）、胆红素（TBIL）、血浆凝血酶原时间（PT）等指标的相关性,并分析其意义。方法 对14例轻度慢性乙型肝炎、13例中度慢性乙型肝炎、8例重度慢性乙型肝炎及22例肝癌患者,应用免疫组化方法分别检测其肝炎组织及肝癌组织内h Fgl 2的表达,使用MIAS医学图像分析软件测定h Fgl 2的表达水平。结果 h Fgl 2在重度慢性乙型肝炎组及肝癌组的表达均高于轻度及中度慢性乙型肝炎组（P均〈0.05）。慢性乙型肝炎组患者肝组织内h Fgl 2蛋白的表达水平与患者血清ALT、AST的水平呈正相关（P〈0.001）,在肝癌组中h Fgl 2的表达水平与患者PT、ALT、AST、TBIL均无相关性（P均〉0.05）。结论 肝癌和重度慢性乙型肝炎患者肝内人纤维介素呈高表达,在慢性乙型肝炎中其水平与肝细胞破坏严重程度相关,而在肝癌中则无相关性,提示人纤维介素在肝癌中的作用可能不同于慢性乙型肝炎。

Hepatic expression of human fibroleukin in patients with liver cancer or hepatitis

Objective To explore the expression of human fibroleukin（hFgl 2）,also known as human fibrinogen-like protein 2 prothrombinase,in the livers of patients with liver cancer or chronic hepatitis B virus （HBV） infection,and to examine possible correlations of this expression with severity of HBV infection and serum biochemistry indicators. Methods Liver tissues were obtained from 35 patients with chronic HBV infection （14 mild, 13 moderate, 8 severe） and 21 patients with liver cancer. Expression of hFgl 2 was detected using immunohistoehemistry,quantified using MIAS medical imaging software and compared among patients with liver cancer or the three severities of viral infection. Results Expression of hFgl 2 was significantly higher in patients with severe chronic HBV infection or liver cancer than in patients with mild or moderate infection. Among chronically infected patients, hFgl 2 expression positively correlated with serum ALT and AST;among patients with liver cancer, hFgl 2 did not correlate with serum ALT,AST,TBIL or PT. Conclusion The protein hFgl 2 is up-regulated in severe chronic HBV infection and in liver cancer. However, the expression level correlated with serum aminotransferase in chronically HBV-infected patients,but not in patients with liver cancer. This suggests that hFgl 2 may play different roles in liver cancer and chronic HBV infection.