男性不明原因不育症患者血浆中AcrAb及Sp17Ab水平变化的研究

目的探讨抗精子蛋白17抗体(Sp17Ab)、抗顶体蛋白酶抗体(AcrAb)在男性不育症发病中的作用。方法采用酶联免疫吸附(ELISA)对12例不明原因不育患者,20例其它原因引起不育的患者及25名正常的生育者的血浆中AcrAb、Sp17Ab进行检测并分析不明原因不育患者血浆中AcrAb和Sp17Ab的水平及其相关性。结果(1)血浆AcrAb水平:不明原因不育患者血浆中顶体蛋白酶抗体(4.15±1.18)μg/L,明显高于对照组的(1.49±0.51)μg/L及其它原因引起的不育(1.56±0.40)μg/L,差异有统计学意义(P0.01);对照组和其它原因引起的不育两者比较,差异无统计学意义(P0.05);(2)血浆Sp17Ab水平:不明原因不育患者血浆中精子蛋白17抗体(8.34±1.88)μg/L,明显高于对照组(4.17±1.07)μg/L及其他原因引起的不育(4.10±0.95)μg/L,差异具有统计学意义(P0.01);对照组和其它原因引起的不育两者比较,差异无统计学意义(P0.05);(3)相关性分析:血浆中顶体蛋白酶抗体与精子蛋白17抗体水平无相关性(r=0.125,P0.05)。结论血浆中顶体蛋白酶抗体、精子蛋白17抗体水平变化与不明原因不育患者发病有关。

The changes of level of anti-sperm protein 17 antibodies and anti-acrosin antibodies in men serum of unexplained infertility

Objective：To study the role of anti-sperm protein 17 antibodies and anti-acrosin antibodies in men patients with infertility.Methods：The levels of AcrAb and Sp17Ab in serum were detected from 12 patients with unexplained infertility,20 patients with infertility for others reason and 25 normal pregnant people who have a child in the past years by enzyme linked immunosorbent assay （ELISA） .Results： （1） The level of AcrAb in serum ：serum level of AcrAb（4.15±1.18）μg/L in unexplained infertility were significant higher than that in control group （1.49±0.51）μg/L and infertility with other reason （1.56±0.40）μg/L,（P〈0.01）;The control group and infertility with other reasons have no significant differences （P〉0.05）;（2） The level of Sp17Ab in serum ：serum level of Sp17Ab in unexplained infertility （8.34±1.88）μg/L were significant higher than that in control group（4.17±1.07）μg/L and infertility with other reason （4.10±0.95）μg /L,（P〈0.01）;The control group and infertility with other reasons have no significant differences （P〉0.05）.（3） The level of AcrAb and Sp17Ab in serum of men have no significant relationship（r=0.125,P〉0.05） Conlusion：The changes of levels of Sp17Ab and AcrAb are related to the occurrence of unexplained infertility.