子宫内膜样腺癌组织中P53、P63和C-erbB2的表达及其意义

背景与目的:p53与C-erbB2是已被证实与子宫内膜样腺癌(endometrioidadenocarcinoma,EC)密切相关的抑癌基因和癌基因,而它们之间的相关性报道较少。p63在结构上与p53高度同源,被认为是p53突变时的一种抑癌基因,它的抑癌特性尚未确定,它在EC中的表达少见文献报道。本研究旨在探讨p53、p63和C-erbB-2基因在EC发生发展中的作用及其与EC临床病理特征的关系。方法:采用免疫组化SP法检测38例EC和23例子宫内膜增生过长(endometrialhyperplasia,EH)及10例正常增生期子宫内膜(benignproliferativeendometrium,BPE)中P53、P63和C-erbB2蛋白的表达情况。结果:(1)P53蛋白在EC组中的阳性率为31.6%,明显高于EH组和BPE组(P<0.05)。P53的表达与EC的手术病理分期和肌层浸润深度有关(P<0.005),而与组织学分级无关(P>0.05)。(2)P63蛋白在EC组中的阳性率为81.6%,与EH组、BPE组比较,差异均有统计学意义(P<0.005)。P63的表达与EC的组织学分级、手术病理分期和肌层浸润深度均无关(P>0.05)。(3)C-erbB2蛋白在EC组中的阳性率为23.2%,与EH组、BPE组比较,差异均无统计学意义(P>0.05)。C-erbB2的表达与EC的手术病理分期和肌层浸润深度有关(P<0.001,P<0.005),而与组织学分级无关(P>0.05)。(4)P53和P63在EC中的表达呈正相关(r=0.443,P<0

Expression of P53, P63, and C-erbB-2 in endometrioid adenocarcinoma and their clinicopathological significance

BACKGROUND &OBJECTIVE: Tumor suppressor gene p53 and oncogene C-e rbB-2 are confirmed to have close relation with endometrioid adenocarcinoma (EC ), few documents have been reported about their correlation. Its structural homo logy to p53, p63 has been considered as a tumor suppressor gene acompany with p5 3 mutation, but its suppressive nature has not been confirmed yet; reports about p63 expression in EC are rare. This study was designed to investigate the roles of p53, p63, and C-erbB2 in tumorigenesis and development of EC, and their cor relation with clinicopathological features of EC. METHODS: Immunohistochemical t echnique was used to detect P53, P63, and C-erbB2 protein expression in 38 case s of EC, 23 cases of endometrial hyperplasia (EH), and 10 cases of benign prolif erative endometrium (BPE) . RESULTS: (1) The positive rate of P53 in EC was 31.6 %,significantly higher than those in EH and BPE (P < 0.05). P53 expression was associated with surgical pathologic stage, and depth of myometrial invasion in E C (P< 0.005), but was not associated with histological grade (P >05). (2) Th e positive rate of P63 in EC was 81.6%, significantly higher than those in EH a nd BPE (P < 0.005). P63 expression was not associated with histological grade, s urgical pathologic stage, and depth of myometrial invasion in EC (P >0.05). (3 ) The positive rate of C-erbB-2 in EC was 23.2%, there was no significant dif ference compared with those in EH or BPE (P >0.05).C-erbB-2 expression was ass ociated with surgical pathologic stage, and depth of myometrial invasion in EC (P< 0.001,P< 0.005),but was not associated with histological grade (P >05) .(4) There was significantly positive correlation between P53 and P63 (r =0.443, P < 0.01)or C-erbB-2 (r =0.490,P < 0.005). CONCLUSIONS: Both p53 and p63 are involved in carcinogenesis of EC; p63 may act as an oncogene in tumorigenesis o f EC. The expression of P53 and C-erbB2 are related to the progression of malig nant EC; P53 and C-erbB-2 co-expression may predict poor prognosis.