甲基莲心碱对氧自由基损伤血管内皮细胞的保护作用

研究了甲基莲心碱（Ｎｅｆ）对外源性氧自由基损伤兔胸主动脉内皮及培养牛胸主动脉内皮细胞的保护作用．结果发现兔胸主动脉环暴露于Ｈ２Ｏ２（０．８ｍｍｏｌＬ－１）／ＦｅＳＯ４（０．２ｍｍｏｌＬ－１）系统３０ｍｉｎ后，乙酰胆碱（ＡＣｈ），二磷酸腺苷及Ａ２３１８７诱导的内皮依赖性舒张反应显著减弱，而硝酸甘油诱导的非内皮依赖性舒张反应不受影响．Ｎｅｆ依剂量性对抗氧自由基所致ＡＣｈ诱导的内皮依赖性舒张反应减弱．培养牛胸主动脉内皮细胞暴露于黄嘌呤／黄嘌呤氧化酶系统４８ｈ后，内皮细胞的生长被抑制６２％，Ｎｅｆ（０．１２５μｍｏｌＬ－１）可使之降为４９％．结果表明Ｎｅｆ对氧自由基所致的血管内皮细胞损伤具有保护作用．

Protective effect of neferine on oxygen free radical-induced damage of vascular endothelial cell

The protective effect of neferine on the injury of rabbit thoracic aortic endothelium and cultured bovine thoracic aortic endothelial cells induced by exogenous oxygen free radicals was investigated. The experimental results showed that after the rings of rabbit thoracic aorta were exposed to H₂O₂ (0.8 mmol·L^-1)/FeSO₄(0.2 mmol·L^-1) system for 30 min, the endothelium- dependent relaxation of rabbit thoracic aortic rings induced by acetylcholine(ACh), ADP or ionophore A23187 was markedly attenuated, but the endothelium-independent relaxation elicited by nitroglycerin was not. Neferine (Nef) dose- dependently reduced the oxygen free radical-induced attenuation of ACh- mediated endothelium-dependent relaxation. The growth of endothelial cells was inhibited by 62％after the bovine thoracic aortic endothelial cells in culture were incubated with xanthine / xanthine oxidase for 48 h. Nef (0.125 μmol·L^-1) could decrease the inhibition rate of endothelial cells to 49%. These results demonstrate that Nef can protect the injury of vascular endothelial cells elicited by oxygen free radical.