氯化镉对小鼠脾细胞增殖与细胞钙稳态关系的影响

以小鼠脾淋巴细胞转化，白介素２（ＩＬ－２）产生，胞内游离钙浓度（［Ｃａ２＋］ｉ），钙调蛋白量为指标，观察氯化镉（ＣｄＣｌ２，１０－２００μｍｏｌＬ－１）对脾细胞增殖的影响与细胞钙稳态的关系．结果表明，在本实验条件下，ＣｄＣｌ２对小鼠脾淋巴细胞转化，ＩＬ－２的产生有明显抑制作用，且与剂量和时间呈依赖关系．ＣｄＣｌ２对脾细胞钙稳态有干扰，致细胞［Ｃａ２＋］ｉ浓度升高，钙调蛋白量下降，也与剂量和时间呈依赖关系．提示ＣｄＣｌ２对小鼠脾细胞增殖的抑制作用与细胞钙稳态失衡密切相关．

Effect of cadmium chloride on the relationship between splenocyte proliferation and calcium homeostasis in mice

In this study, mouse splenocytes were used to determine the effects of CdCl₂(10-200 μmol·L^-1) on lymphocyte transformation, interleukin 2 (IL-2) production, intracellular free Ca²⁺ concentration (［Ca²⁺］_i), and calmodulin activity. The results showed that CdCl₂ inhibited lymphocyte transformation and IL-2 production with a significant linear dose and time response relationship. CdCl₂ also significantly enhanced mouse splenocyte ［Ca²⁺］_i and reduced calmodulin activity with a linear dose and time-response relationship. This study demonstrated a strong association between the inhibition of splenocyte proliferation and changes in calcium homeostasis caused by Cd.