白芍总甙对大鼠腹腔巨噬细胞Ca2+跨膜转运的影响

通过检测大鼠腹腔巨噬细胞(M_ф

Effect of total glucosides of paeony on calcium ion transport across membrane of peritoneal macrophages in rats

Effect of different concentrations of total glucosides of paeony (TGP) on calcium ion across membrane of rat peritoneal macrophages (PM_ф) were studied by measuring cytoplasmic free Ca²⁺ concentration (［Ca²⁺］_i) and ⁴⁵Ca fluxes in PM_ф from rats. The results showed that TGP (2.5-20 mg·L^-1) had a marked enhancement on elevated ［Ca²⁺］_i in PM_ф stimulated by the suboptimal concentrations of calcimycin(0.1 μmol·L^-1), lipopolysaccharide (LPS, 5 mg·L^-1) or formyl-methionine-leucine- phenylalanine (fMLP, 1 μmol·L^-1), in the presence of extracellular Ca²⁺ (1 mmol·L^-1), but TGP did not have any effect on increased ［Ca²⁺］_i through releasing Ca²⁺ in its internal reservoir of PM_ф induced by LPS or fMLP. TGP could increase ⁴⁵Ca influx, but its higher concentrations (10-40 mg·L^-1) could pro-mote ⁴⁵Ca efflux. These results sugges that TGP can increase the influx of extracellular calcium, and that the higher concentration of TGP can promote efflux of intracellular calcium and then down-reglate the intracellular calcium concentration.