肿瘤坏死因子α对妊娠早期人滋养层细胞分泌绒毛膜促性腺激素的调节机理研究

从正常妊娠早期人胎盘中分离出滋养层细胞，经重组肿瘤坏死因子α（ｒＴＮＦ－α）刺激，ＭＴＴ生物检测法测定其上清液白细胞介素６（ＩＬ－６）的活性，同时用酶免疫分析法检测人绒毛膜促性腺激素（ｈＣＧ）的活性。结果显示，被ｒＴＮＦ－α刺激之后，合体滋养层细胞分泌ＩＬ－６先于ｈＣＧ．当用抗ＩＬ－６受体的单克隆抗体ＰＭ－１阻断后，ｈＣＧ分泌量明显下降，而此时ＩＬ－６的产生量不受影响。当滋养层细胞经ｒＴＮＦ－α刺激产生ＩＬ－６之后再用ＰＭ－１阻断ＩＬ－６受体，则不影响ｈＣＧ的分泌。结果表明，肿瘤坏死因子刺激合体滋养层细胞先产生ＩＬ－６，然后再通过其ＩＬ－６受体信号系统调节ｈＣＧ的分泌。

Study of the regulatory mechanism of action of tumour-necrosis factor-αon theroduction of hCG by human early pregnancy trophoblast cells

bjective:To investigate the regulatory effect of tumornecrosis factor-α(TNF -α)on th production of hCG by huma trophoblast cells . Design :An in vitro experimental study . etting:Laboratory in Osaka University Medical School . Material: Trophoblast cells were isolated from human placenta of early pregnancy Recombinant TNF-α,IL-1,IL-6 and IL-6 receptor monoclonal antibody PM-1 were obtained from Japan . Methods: Trophoblast cclls were isolated from hunad placenta of early pregnancy with percoll methodand incubated with rTNF-α hCG concentration in the medium was measured with EIA, and IL-6 activity was measured by MTT method. Results:,stimu1ation with rTNF-α the trophot cells secreted IL-6 earlier than hCG.When blocked with the monoclonal antibody of IL-6 receptor ,PM-1, hCG secretion significantly decreased,but the IL-6 production is not affected. If rTNF-α stimulated the production of IL-6 befre the use of PM-1 to block the IL-6 receptor ,then the secretion of hCG was not affected.concl usion :The results indicated that rTNF -α stimulated the trophoblast cells to produce IL-6,and through the Il-6 receptor system regulate the hCG secretion.